Center for Mass Spectrometry and Proteomics
The Center for Mass Spectrometry and Proteomics (CMSP) facility is a subunit of the Department of Biochemistry, Molecular Biology and Biophysics (BMBB). The CMSP provides support, equipment, and expertise for analyzing complex protein mixtures. Mass spectrometric data can provide intact protein molecular weight as well as protein identification by peptide mass fingerprint or MS/MS data, combined with protein database searching. In addition, some mass spectrometers provide accurate mass measurement for organic molecules and relative protein quantification via isotope labeling techniques (e.g., iTRAQ™).
The center also has equipment to help with complex protein separation and differential expression of protein between two samples. The facility has the capability of traditional 2D SDS-PAGE, DIGE and high throughput spot picking, digestion, and desalting.
Publications with CMSP authorship, acknowledgement or instrument usage
Next Proteomics Workshop: March 18 - 20, 2014 (click here)
The workshops involve lectures, hands-on instrument experience and discussions of critical aspects of MS-baed proteomic analyses, including: experimental design, sample preparation, protein preparation for MS identification, liquid chromatography, mass spec data acquisition and mass spec data analysis. Workshops include practical applications of gel-based protein separation, including 1D & 2D gel separation, DIGE technology and non-gel based protein preparation. Individuals will observe MS data acuisition on a variety of instruments with multiple ionization sources (MALDI, ESI) and multiple scan modes (MS, MS/MS, MSn).