Robert Brambl
 Professor, Department of Plant Biology
Ph.D., University of Nebraska, 1970
Molecular and Cellular Biology
Contact Information
Mailing Address:
Dr. Robert Brambl
Department of Plant Biology
University of Minnesota
250 Biological Sciences Center
1445 Gortner Ave.
St. Paul, MN 55108
Office: 638 Biological Sciences Center
Phone: 612-625-7080
Fax: 612-625-1738
E-mail: brambl@umn.edu
Research Interests
Our laboratory is using cells of Neurospora crassa and techniques of
genetics, biochemistry, and molecular biology to study the regulation
of mitochondrial gene expression at the level of translation. We wish
to identify nucleus-encoded proteins that are involved in the
recruitment of specific mRNAs into the mitochondrial translational
system. We also are interested in the synthesis and assembly of
protein components of the mitochondrial respiratory membrane and in
post-translational modifications of these mitochondrial proteins,
such as fatty acylation, that may be involved in their enzymatic
function. In another project in our laboratory, we are studying the
the cellular localization and function of the heat shock proteins, or
chaperones, and the role of the heat shock response in protecting
cells against physical stress. We are identifying cellular components
with which these chaperones interact, and by targeted gene
disruption, we are determining the contributions of these chaperones
to organelle assembly and metabolism in normal and stressed cells.
Selected Publications
Bittner-Eddy, P., Monroy, A., and Brambl, R. (1994) Expression of mitochondrial genes in the germinating conidia of Neurospora crassa. J. Mol. Biol. 235: 881-897.
Plesofsky-Vig, N. and Brambl, R. (1995) Disruption of the gene for
hsp30, an a-crystallin-related heat shock protein of Neurospora
crassa, causes defects in thermotolerance. Proc. Natl. Acad. Sci., USA 92: 5032-5036.
Vassilev, A. O., Plesofsky-Vig, N., and Brambl, R. (1995) Cytochrome c oxidase in Neurospora crassa contains myristic acid covalently linked to subunit 1. Proc. Natl. Acad. Sci., USA 92: 8680-8684.
Plesofsky-Vig, N. and Brambl, R. 1998. Characterization of an 88-kDa heat shock protein of Neurospora crassa that interacts with Hsp30. J. Biol. Chem. 273: 11335-11341.
Plesofsky, N. and Brambl, R. 1999. Glucose metabolism in Neurospora is altered heat shock and by disruption of HSP30. Biochim. Biophys. Acta 1449:73-82.
Plesofsky, N., Gardner, N., Lill, R., and Brambl, R. 1999. Disruption of the gene for Hsp30, an alpha-crystallin-related heat shock protein of Neurospora crassa, causes defects in import of proteins into mitochondria. Biol. Chem. 380: 1231-1236.
Plesofsky, N., Gardner, N., Videira, A, and Brambl, R. 2000. NADH
dehydrogenase in Neurospora crassa contains myristic acid covalently linked to the ND5 subunit peptide. Biochim. Biophys. Acta 1495: 223-230.
Plesofsky, N. and Brambl, R. 2002. Analysis of interactions between domains of a small heat shock protein, Hsp30, of Neurospora crassa. Cell Stress & Chaper, 7:374-386.
Kleidon, J., Plesofsky, N., and Brambl, R. 2003. Transcripts and transcript-binding proteins in mitochondria of Neurospora crassa. Mitochondrion 2:345-360.
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