Main Phone:

Bruce Witthuhn
Research Assistant Professor

Expertise
*2D electrophoresis and DIGE labeling of proteins
*ProteomeLab (HPLC separation of Proteins)
*Protein isolation and purification
*Bacterial culture
*Tissue and cell culture
*Protein isolation and purification
*General molecular biology, protein separation and   recombinant DNA techniques

Witthuhn BA and Bernlohr DA (2001) Upregulation of the Bone Morphogenetic Protein GDF-3/Vgr-2 in Adipose Tissue of FABP4 Null Mice. Cytokine 14(3): 129-135

Witthuhn BA, Williams MD, Kerawalla H, Uckun FM, (1999) Differential substrate recognition capabilities of Janus family protein tyrosine kinases within the interleukin 2 receptor (IL2R) system. Leuk Lymphoma 32; 289-297.

Feng J, Witthuhn BA, Matsuda T, Kohlhuber F, Kerr IM, and Ihle JN, (1997) Activation of Jak2 catalytic activity requires phosphorylation of Y-1007 in the kinase activation loop, Molec Cell Biol 17: 2497-2501.

Witthuhn, BA, and Faust, C, (1996) Isolation and characterization of the rat b -subunit gene of the high affinity receptor for immunoglobulin e , Mol Immun . 33: 881-890.

Nosaka T, van Deursen J, Tripp R, Witthuhn BA, McMickle AP, Doherty PC, Grosveld G., and Ihle JN, (1995) Defective lymphoid development in mice lacking Jak3,   Science 270: 800-802.

Witthuhn, BA, and Faust C, (1995) Tissue specific expression in mouse P815 mastocytoma cells of the cloned rat a -subunit gene of the high affinity receptor for Ig e , Immunol Lett 47: 39-43.

Miyazaki T, Kawahara A, Minami Y, Liu Z, Witthuhn BA, Silvennoinen O, Ihle JN, and Taniguchi T, (1994) IL-2-induced activation of Jak1 and Jak3 PTKs via selective association with the IL-2R b and IL-2R g chains, Science 266: 1045-1047.

Witthuhn BA, Silvennoinen O, Miura O, Lai KL, Cwik C, Liu ET, and Ihle JN,   (1994) Involvement of the Jak-3 Janus kinase in signaling by interleukins 2 and 4 in lymphoid and myeloid cells, Nature 370: 153-157.

Witthuhn BA, Quelle FW, Silvennoinen O, Yi T, Tang B, Miura O, and Ihle JN, (1993) Jak2 associates with the erythropoietin receptor and is tyrosine phosphorylated and activated following stimulation with erythropoietin, Cell 74: 227-236.