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Services and Procedures
Simple Protein Identification
| Step 1 |
Step 2 |
Step 3 |
Steps 4 |
Step 5 |
Protein Isolation
(e.g. SDS-PAGE),
Protein Purification |
Proteolytic
Digestion |
Desalt Peptide Mixture |
RP C18 ZipTip (Millipore)
or
|
MALDI-TOF MS,
MALDI-TOF MS/MS or
NanoESI infusion MS/MS
|
+ separation
-> RP C18 HPLC
or
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Micro-ESI-MS/MS
|
| RP Poros R2 (ABI) |
Micro-ESI-MS/MS
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RP = reversed phase
ESI = electrospray
MALDI = Matrix Assisted Laser Desorption Ionization
MS = mass spectrometry
HPLC = High performance liquid chromatography
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