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Differential Protein Expression

2D gels -> DIGE or IEF PAGE -> Probot or Proprep ->MALDI or ESI
Eprogen

ICAT and ITRAQ description

ICAT - Method to label cysteines for protein relative abundance quantitation
ITRAQ - Method to label primary amines for protein abundance quantitation
ITRAQ - ABI Application Note - ITRAQ TM Reagents

Complex Protein Mixture

MALDI -> Protein digest -> Probot 1D, or Probot 2D

ESI -> Protein digest -> HPLC 1D, or off line cation 1D HPLC

Protein Modifications

iTRAQ

qTRAP

Intact Protein MS

Cleanup Poros -> MALDI or ESI

ZT -> MALDI or ESI

Digestion Protocols

In gel digest

In solution digest

Data Analysis

Mascot

ABI software