The Center for Mass Spectrometry and Proteomics facility provides support, equipment, and expertise for analyzing complex protein mixtures. Mass spectrometric data can provide intact protein molecular weight as well as protein identification by peptide mass fingerprint or MS/MS data, combined with protein database searching. In addition, some mass spectrometers provide accurate mass measurement for organic molecules and relative protein quantification via isotope labeling techniques (e.g., iTRAQ™).

The center also has equipment to help with complex protein separation and differential expression of protein between two samples. The facility has the capability of traditional 2D SDS-PAGE, DIGE, PF (2D liquid chromatofocusing) and high throughput spot picking, digestion, and desalting.

Advisory Committee

Support

• NSF Grant 9871237: Mass Spectrometry for Biological Sciences
  (Dr. Gary Nelsestuen, P.I.)
• NSF Grant CHE0078192: Data System Upgrades for Mass Spectrometry
• NIH Grant RRR15808: Quadrupole Time of Flight Mass Spectrometry
• The College of Biological Sciences
• The College of Food, Agriculture and Natural Sciences
• The Minnesota Agricultural Experiment Station
• The University of Minnesota Graduate School
• Office of the Executive Vice President and Provost
• Office of the Senior Vice President for the Academic Health Center
• The State of Minnesota
• NSF Major instrumentation grant 0215759 "Acquisition of Instrumentation for
  a Proteome Analysis Core" (D. Bernlohr, P.I.)
• NSF instrumentation grant (V. Kapur, P.I.)