16S rRNA structure
http://www.biochem.umd.edu/biochem/kahn/bchm465-01/ribosome/16SrRNA.html
The structure of the ribosome was recently solved. Because of the importance of the 16S rRNA subunit for microbial taxonomic research, it is valuable to see the stucture of this component in isolation.
Wikipedia, 16S ribosomal RNA
http://en.wikipedia.org/wiki/16S_ribosomal_RNA
This site provides an excellent overview on the biology of 16S rRNA, the uses of the sequence, and methods for PCR amplification of the genes.
16S rRNA gene sequencing: Identification of Bacillus anthracis
http://www.cdc.gov/ncidod/EID/vol8no10/02-0391.htm
This webpage of the Center for Disease Control illustrates a practical use of 16S rRNA gene sequencing, in this case to differentiate Bacillus anthracis from non-pathogenic Bacillus strains.
Green genes: 16S rRNA gene database and workbench
http://greengenes.lbl.gov/cgi-bin/nph-index.cgi
Alignments of 16S rRNA gene sequences provides important phylogenetic information. This site provides these alignments and data that can be browsed or searched in many ways.
Prokaryotic 16S rRNA signature database
http://prion.bchs.uh.edu/16S_signatures/
This database of 16S rRNA signature sequences currently contains 228,905 undecamers.
Structure of the 16S ribosomal RNA
http://helix-web.stanford.edu/ribo.html
This site describes a model of the 16S rRNA structure obtained from hydroxyl footprinting data.
Ribosomal database project II
http://rdp.cme.msu.edu/
The ribosomal database provides sequence data, aligned sequences and phylogenetic data. These are provded freely to the international scientific community.
BioInfoBank Library: 16S ribosomal RNA
http://lib.bioinfo.pl/meid:966
This site provides an annotated bibliography for papers dealing with 16S rRNA data and genetics.
Laboratory class: Sequencing RNA gene to identify bacteria
http://srv2.lycoming.edu/~newman/rrna/rrnalab.htm
This site describes a convenient undergraduate laboratory exercise in which students obtain 16S rRNA gene sequence data and compare sequences from unknown bacteria with those in the Ribosomal Database.
ReNaBi: 16S rDNA bacterial identification
http://www.renabi.fr/article612.html
This page of a bioinformatics group provides links to many resources dealing with 16S rRNA sequences.
SIMO: 16S rRNA database
http://simo.marsci.uga.edu/public_db/
This page of the Sapelo Island Microbial Observatory allows searching against a set of environmentally-derived bacterial 16S rRNA sequences.
BIOLOG versus 16S rRNA identification of bacteria
http://www.osti.gov/bridge/purl.cover.jsp?purl=/754656-NGcbMM/native/
The webpage provides a link to a web article describing a comparison of the BIOLOG
identification method with 16S rRNA sequence data for identifying bacteria isolated from
the deep subsurface.
E. coli 16S rRNA gene products
http://ecoliwiki.net/colipedia/index.php/16S_rRNA
Information and links are provided for Escherichia coli 16S rRNA gene products.
Human oral microbiome 16S rRNA database
http://www.homd.org/modules.php?name=Article&file=index&sid=28
This database contains a comprehensive set of 16S rRNA sequences for all of the major human oral microbial taxonomic groups.
Hypervariable regions in 16S rRNA sequences
http://www.bioinformatics-toolkit.org/Help/Topics/hypervariableRegions.html
This site illustrates the observation that there are regions of much greater variability in 16S rRNA sequences and that less variable regions reflect the functional importance of these conserved regions.
Phylogeny tutorials: 16S rRNA multiple sequence alignments
http://edzna.ccg.unam.mx/rhizobial-taxonomy/node/7
This site provides an excellent step-by-step tutorial for using 16S rRNA sequence data for phylogenetic analysis.