DNA

• Mini-Prep: Plasmid isolation from bacteria.
• Preperation of a DNA probe
• Screening a cDNA library.
• Extracting gDNA from adult tail fins.

RNA

• Total RNA Extraction
• Northern Blots
• Gel Electrophoresis of RNA
  • Glyoxal Gel Electorphoresis

Protein

• Immunofluorescence
• Bgal Assays
  • Tropix Chemiluminescence
  • Bgal Staining Whole Mount

Reporter Gene In Vivo Staining

Polymerase Chain Reaction (PCR)

• PCR-general
• Bacterial Colony PCR: Allows quick identification of correct clones if primers exist that allow clone identification by size or hybridization.

SBST Bibliography

• PDF listing

ProTIS for SB Transposons

• instructions
• ProTIS.pl