CHAODONG WU
Research Experience

9/95 - 7/98

  1. Successfully cloning and sequencing nearly full-length E2/NS1 gene of genotype III/2a Chinese isolates of hepatitis C virus (GenBank accession number: AF023154)

  2. Successfully expressing E2 glycoprotein of genotype III/2a Chinese isolates of hepatitis C virus in mammalian cells for diagnostic value or vaccine development.

  3. Effectively detecting anti-E2 antibodies with recombinant E2 glycoprotein in hepatitis C patients

  4. Successfully inducing anti-E2 antibody in mice by immunization of plasmid containing coding sequences for E2 as immunotherapy or gene therapy strategies

  5. Construction of recombinant adenovirus for expression E2 glycoprotein

  6. Signal transduction study on mitogen-activated protein kinase (MAPK) and p34cdc2 kinase

9/92 - 7/95

  1. establishment animal model of endotoxin induced acute liver injury

  2. detection of tumor necrosis factor-alpha and interleukin 6 in animal model

  3. histology and immuohistochemistry analysis of liver

  4. analysis of prevention and treatment effects of drug on acute liver injury

PUBLICATIONS

  1. Wu CD, Li MZ, Zhang YP, et al. Effects of Reduqing injection on plasma tumor necrosis factor-alpha and interleukin-6 levels in rabbits with endotoxin-induced DIC. Chinese Journal of Integrated Traditional and Western Medicine,1995;15:356-358.( in Chinese with English abstract)

  2. Wu CD, Li MZ, Chen CL, et al. Endotoxin-induced liver injury and plasma tumor necrosis factor-alpha, interleukin-6 level changes in rabbits. Chinese Journal of Digestion, 1995;15:256-258. (in Chinese with English abstract)

  3. Wu CD, Li MZ, Chen CL, et al. Endotoxin-induced liver injury and changes in the level of plasma tumor necrosis factor-alpha and interleukin-6 in rabbits. Chin Med J,1995;108:548-550. (in English )

  4. Wu CD, Li MZ, Zhang MF, et al. Effects of Traditional Chinese medicine reduqing in interleukin-6 and acute phase proteins in rabbits with endotoxin-induced disseminated intravascular coagulation. Chinese Critical Care Medicine, 1996;8:3-4. (in Chinese with English abstract)

  5. Wu CD, Tao QM. Homology of E2/NS1 gene derived from genotype III Chinese isolates of hepatitis C virus to that from reported isolates. International Symposium of Hepatology, August 12-15,1997. Beijing, China. S132

  6. Wu CD, Tao QM. Cloning and sequencing of E2/NS1 gene derived from genotype III Chinese isolates of hepatitis C virus. Natl Med J China, 1998;78:115-117. (in Chinese with English abstract)

  7. Wu CD, Tao QM. Comparison between homologies of E2/NS1 gene from genotype III Chinese isolates of hepatitis C virus and that from reported isolates. Chin Med J, 1998;111:807-809. (in English)

  8. Wu CD, Tao QM. Homology of E2/NS1 gene derived from genotype III Chinese isolates of hepatitis C virus to that from reported isolates. Chin Biochem J, in press. (in Chinese with English abstract)

  9. Wu CD, Gao JE, Tao QM. Stable expression E2 glycoprotein of hepatitis C virus in mammalian cell. Chin Biochem J, 1998;14:15-19. (in Chinese with English abstract)

  10. Wu CD, Tao QM. Expression E2 glycoprotein of genotype III Chinese isolates of hepatitis C virus and detection antibodies against E2 in hepatitis C patients. Chin J Exp Clin Virol, in press. (in Chinese with English abstract)

  11. Wu CD, Tao QM. E2 glycoprotein of genotype III Chinese isolates of hepatitis C virus expressed in mammalian cell as antigen for anti-E2 detection. Chin Med Sci J, 1998:13:77-79. (in English)

  12. Wu CD, Tao QM. Antibodies response to E2 glycoprotein induced in mice by immunization of plasmid DNA containing sequence derived from genotype III Chinese isolates of hepatitis C virus coding for E2. Chin Med J, in press. (in English)

  13. Cheng Y, Zheng FD, Wu CD, Tang CS. Effect of Trapidil on expression and activities of mitogen-activated protein kinase (MAPK) and p34cdc2 kinase in cultured rat aortic smooth muscle cells. Acta Pharmacologica Sinica 1998, in press. (in English)