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Table 4. Insertion sequence (IS) elements in K12 strains
|
Fragments detected |
Elements in strains |
|||||||||||
|
A side |
B side |
|||||||||||
|
Element |
Start, bp |
End, bp |
Flanking genes |
Direction |
Fragment size |
Comigrant* |
Fragment size |
Comigrant* |
MG1655 |
CGSC6300 |
TD2 |
TD10 |
|
IS1-1 |
19811 |
20508 |
b0020-b0023 |
- |
272 |
199 |
IS1-2 |
P† |
P |
P |
P |
|
|
IS1-2 |
278402 |
279099 |
b0263-b0266 |
- |
174 |
191 |
IS1-1 |
P |
P |
P |
P |
|
|
IS1-3 |
289873 |
290570 |
b0273-b0276 |
- |
171 |
IS1-2 |
577 |
P |
P |
P |
P |
|
|
IS1-4 |
1049056 |
1049753 |
b0987-b0989 |
+ |
281 |
IS1-1 |
139 |
P |
P |
P |
P |
|
|
IS1-5 |
1976542 |
1977239 |
b1892-b1895 |
- |
452 |
226 |
P |
P |
||||
|
IS1-6 |
3581114 |
3581811 |
b3443-b3446 |
+ |
712 |
1020 |
P |
P |
P |
P |
||
|
IS1-7 |
4516095 |
4516370 |
b4293-b4295 |
+ |
1354 |
575 |
IS1-3 |
P |
P |
P |
P |
|
|
IS2-1 |
380530 |
381803 |
b0359-b0362 |
+ |
308 |
841 |
P |
P |
P |
P |
||
|
IS2-2 |
1465945 |
1467218 |
b1401-b1405 |
- |
194 |
203 |
IS2-4 |
P |
P |
P |
P |
|
|
b1579 transposase |
1649536 |
1650732 |
b1578-b1580 |
+ |
778 |
P |
P |
P |
P |
|||
|
IS2-3 |
2066974 |
2068247 |
b1995-b1998 |
- |
287 |
IS2-1 |
444 |
P |
P |
P |
P |
|
|
IS2-4 |
2994394 |
2995622 |
b2859-b2862 |
- |
589 |
219 |
P |
P |
P |
P |
||
|
IS2-5 |
3184203 |
3185431 |
b3043-b3046 |
+ |
410 |
672 |
P |
P |
P |
P |
||
|
IS2-6 |
4495795 |
4497068 |
b4271-b4274 |
+ |
204 |
IS2-2 |
163 |
P |
P |
P |
P |
|
|
IS3-1 |
390963 |
391829 |
b0371-b0373 |
- |
489 |
IS3-4 |
142 |
P |
P |
P |
P |
|
|
IS3-2 |
566361 |
567227 |
b0540-b0542 |
+ |
787 |
346 |
P |
P |
P |
P |
||
|
IS3-3 |
1093498 |
1094364 |
b1025-b1027 |
- |
1009 |
772 |
P |
P |
P |
P |
||
|
IS3-4 |
2168554 |
2169420 |
b2088-b2090 |
+ |
502 |
842 |
P |
P |
P |
P |
||
|
IS3-5 |
314811 |
315677 |
b0298-b0300 |
+ |
863 |
158 |
IS2-1 |
P |
P |
P |
P |
|
|
IS4 |
4499671 |
4500999 |
b4277-b4279 |
- |
629 |
450 |
P |
P |
P |
P |
||
|
IS5-1 |
273325 |
274341 |
b0258-b0260 |
- |
489 |
IS5-5 |
497 |
P |
P |
P |
P |
|
|
IS5-2 |
573960 |
574976 |
b0551-b0553 |
- |
590 |
1605 |
P |
P |
P |
P |
||
|
IS5-3 |
687220 |
688236 |
b0655-b0657 |
- |
348 |
420 |
IS5-8 |
P |
P |
P |
P |
|
|
IS5-4 |
1394100 |
1395116 |
b1330-b1332 |
+ |
569 |
IS5-2 |
471 |
IS5-1 |
(P)‡ |
(P) |
(P) |
(P) |
|
IS5-5 |
1425770 |
1426750 |
b1369-b1371 |
- |
491 |
1543 |
P |
P |
P |
P |
||
|
IS5-6 |
2064327 |
2065343 |
b1993-b1995 |
- |
747 |
1210 |
P |
P |
P |
P |
||
|
IS5-7 |
2099917 |
2100933 |
b2029-b2031 |
- |
306 |
303 |
P |
P |
P |
P |
||
|
IS5-8 |
2287085 |
2288101 |
b2191-b2193 |
- |
314 |
IS5-7 |
411 |
P |
P |
P |
P |
|
|
IS5-9 |
3128193 |
3129209 |
b2981-b2983 |
+ |
1085 |
672 |
P |
P |
P |
P |
||
|
IS5-10 |
3363337 |
3364353 |
b3217-b3219 |
- |
207 |
1126 |
P |
P |
P |
P |
||
|
IS5-11 |
3649812 |
3650828 |
b3504-b3506 |
- |
734 |
IS5-6 |
1335 |
P |
P |
P |
P |
|
|
IS30-1 |
269827 |
270978 |
b0255-b0257 |
+ |
720 |
314 |
P |
P |
P |
P |
||
|
IS30-2 |
1467382 |
1468533 |
b1401-b1405 |
+ |
305 |
297 |
P |
P |
P |
P |
||
|
IS30-3 |
4505034 |
4506185 |
b4283-b4285 |
- |
405 |
766 |
P |
P |
P |
P |
||
|
Intergenic Region |
279155 |
279328 |
b0265-b0266 |
254 |
P |
P |
P |
P |
||||
|
IS150 |
3718309 |
3719678 |
b3556-b3559 |
+ |
195 |
176 |
P |
P |
P |
P |
||
|
IS186-1 |
15445 |
16557 |
b0015-b0017 |
+ |
503 |
1501 |
P |
P |
P |
P |
||
|
IS186-2 |
607288 |
608400 |
b0581-b0583 |
+ |
2204 |
417 |
P |
P |
P |
P |
||
|
IS186-3 |
2512345 |
2513463 |
b2393-b2395 |
+ |
764 |
667 |
P |
P |
P |
P |
||
|
Additional Elements |
||||||||||||
|
IS1-a |
257908 |
b0240 |
- |
1488 |
738 |
P |
||||||
|
IS1-b |
335460 |
b0319-b0326 |
- |
609 |
309 |
P |
||||||
|
IS1-c |
1871063 |
b1786 |
- |
1211 |
484 |
P |
||||||
|
IS1-d |
2037484 |
b1970-b1971 |
+ |
617 |
IS1-b |
1066 |
P |
P |
||||
|
IS1-e |
2623548 |
b2502 |
+ |
282 |
IS1-4 |
368 |
P |
P |
||||
|
IS1-f |
2768501 |
b2635 |
+ |
1608 |
544 |
P |
P |
P |
||||
|
IS1-g |
3275070 |
b3130 |
+ |
529 |
2117 |
P |
P |
|||||
|
IS1-h |
4539642 |
b4313 |
- |
175 |
IS1-2 |
1119 |
P |
P |
||||
|
IS2-a |
1588558 |
b1506 |
+ |
765 |
717 |
P |
P |
|||||
|
IS2-b |
2927499 |
b2796 |
+ |
138 |
348 |
P |
P |
|||||
|
IS3-a |
331175 |
b0314-b0315 |
+ |
1002 |
IS3-3 |
563 |
P |
|||||
|
b0805 region |
838781 |
b0805 |
+ |
530 |
P |
P |
P |
|||||
|
IS3-c |
2460317 |
b2351 |
+ |
489 |
IS3-4 |
1642 |
P |
P |
||||
|
IS3-d |
3427623 |
b3280 |
- |
1004 |
553 |
IS3-a |
P |
P |
||||
|
IS3-e |
3776882 |
b3604 |
- |
1435 |
935 |
IS3-e |
P |
P |
P |
|||
|
IS3-f |
4466303 |
b4242 |
- |
640 |
935 |
IS3-f |
P |
P |
P |
|||
|
IS5-a |
1102866 |
b1040 |
+ |
365 |
400 |
P |
P |
|||||
|
IS30-a |
2246187 |
b2156 |
- |
303 |
IS30-2 |
445 |
P |
P |
P |
|||
|
IS30-b |
4115565 |
b3927 |
- |
692 |
186 |
P |
P |
|||||
|
IS186-a |
4541184 |
b4314 |
- |
606 |
534 |
P |
P |
|||||
*C
omigrants are fragments from other ISs that comigrate with the row IS fragment.†
P, present.‡
IS5-4 was detected by convensional PCR of genomic DNA (gDNA) because both its fragments are masked by comigrants from other ISs.|
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