What's Inside

Berman Lab

Lab Safety

Standard Operating Procedures & Chemical Hygiene Plan

Chemical Hazards


PHENOL

  • Gloves must be worn during handling.
  • This is a carcinogen. Dispose in provided container.
  • Whenever possible, perform procedures in fume hood.
  • In case of accident, wash exposed skin with water for at least 15 minutes.
  • Accidental Spills: Only try to clean up small spills. Wear gloves and use instant absorbent powder. Clean area with paper towels and then dispose of them in the provided container.
  • In case of large spill, evacuate lab and call 6-6002 or 911.

Hydroxyurea

  • Ribonucleotide reductase inhibitor. Toxic agent. May cause hereditable genetic damage.
  • Quantities used range from 0.2g to 4g per experiment (dissolved in yeast growth medium).
  • Gloves must be worn during handling.
  • Accidental exposure procedure: Wash exposed skin thoroughly.
  • Accidental spill: Wear gloves while cleaning up spill.

Ethidium bromide

  • Agarose DNA gels. Toxic agent. Mutagen. Milligram quantities per gel.
  • Gloves must be worn during handling.
  • Use fume hood when weighing or handling powder.
  • Dispose of in ethidium bromide waste container (gels).
  • Accidental exposure procedure: Wash exposed skin thoroughly.
  • Accidental spill: Wear gloves while cleaning up spill.

Formaldehyde

  • Used for fixation of yeast. Toxic agent. Avoid skin exposure/inhalation.
  • Quantities used range from 1 ml to 10 ml per experiment (37% solution). If larger quantities are required, or if procedure is repeated frequently outside of a fume hood, exposure monitoring may become necessary.
  • Gloves must be worn during handling.
  • Whenever possible, perform procedures in fume hood.
  • Collect formaldehyde waste in container provided.
  • Accidental exposure procedure: Wash exposed skin thoroughly.
  • Accidental spill: Only attempt to clean up small spills (1-5 ml): Wear gloves, safety glasses and face mask.
  • Larger spills: Evacuate lab, commence emergency procedure.

Recombinant DNA Hazards

Wear gloves while handling yeast genomic library DNA. Quantities used are in the 0.1 - 2 ul range. Spill risk is negligible.