
Lab Safety
Standard Operating Procedures & Chemical Hygiene Plan
Chemical Hazards
PHENOL
- Gloves must be worn during handling.
- This is a carcinogen. Dispose in provided container.
- Whenever possible, perform procedures in fume hood.
- In case of accident, wash exposed skin with water for at least 15 minutes.
- Accidental Spills: Only try to clean up small spills. Wear gloves and use instant absorbent powder. Clean area with paper towels and then dispose of them in the provided container.
- In case of large spill, evacuate lab and call 6-6002 or 911.
Hydroxyurea
- Ribonucleotide reductase inhibitor. Toxic agent. May cause hereditable genetic damage.
- Quantities used range from 0.2g to 4g per experiment (dissolved in yeast growth
medium).
- Gloves must be worn during handling.
- Accidental exposure procedure: Wash exposed skin thoroughly.
- Accidental spill: Wear gloves while cleaning up spill.
Ethidium bromide
- Agarose DNA gels. Toxic agent. Mutagen. Milligram quantities per gel.
- Gloves must be worn during handling.
- Use fume hood when weighing or handling powder.
- Dispose of in ethidium bromide waste container (gels).
- Accidental exposure procedure: Wash exposed skin thoroughly.
- Accidental spill: Wear gloves while cleaning up spill.
Formaldehyde
- Used for fixation of yeast.
Toxic agent. Avoid skin exposure/inhalation.
- Quantities used range from 1 ml to
10 ml per experiment (37% solution). If larger quantities are required, or if
procedure is repeated frequently outside of a fume hood, exposure monitoring
may become necessary.
- Gloves must be worn during handling.
- Whenever possible, perform procedures
in fume hood.
- Collect formaldehyde waste in container provided.
- Accidental exposure procedure: Wash exposed skin thoroughly.
- Accidental spill: Only attempt to clean up small spills (1-5 ml): Wear gloves, safety glasses and face mask.
- Larger spills: Evacuate lab, commence emergency
procedure.
Recombinant DNA Hazards
Wear gloves while handling yeast genomic library DNA. Quantities used are in the 0.1 - 2 ul range. Spill risk is negligible.