C. Albicans Fluorescent Protein Cassettes >

C-terminal Fluorescent Protein Constructs

Candida Fluorescent Protein Cassettes
as described in Gerami-Nejad et al., Yeast: 18(9):859-864

Berman plasmids that are available through the Fungal Genetics Stock Center

Please note:

• The optimal PCR reaction concentration of the primers listed in the above article is 500nM or greater.
• The excitation wavelength of CFP is 420-450 nm, NOT 380-450 nm as stated in the above article.
• Maps not to scale. Click on maps to see sequence.

1: Primers

forward:

GGTGGTGGTTCTAAAggTgAAgAATTATT

reverse:

GAATTCCGGAATATTTATGAGAAAC

2: Primers

forward:

GGTGGTGGTTCTAAAggTgAAgAATTATT

reverse:

TCTAGAAGGACCACCTTTGATTG

3: Primers

forward:

GGTGGTGGTTCTAAAggTgAAgAATTATT

reverse:

GAATTCCGGAATATTTATGAGAAAC

4: Primers

forward:

GGTGGTGGTTCTAAAggTgAAgAATTATT

reverse:

TCTAGAAGGACCACCTTTGATTG

5: Primers

forward:

GGTGGTGGTTCTAAAggTgAAgAATTATT

reverse:

GAATTCCGGAATATTTATGAGAAAC

6: Primers

forward:

GGTGGTGGTTCTAAAggTgAAgAATTATT

reverse:

TCTAGAAGGACCACCTTTGATTG