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Director
Mark A. Sanders

Staff
Tracy E. Anderson
Gail Celio

Students
Chemi Banari
Alex Cramer
Anna Hottinger
Alex Schauer
Ashleigh Skaalen

University of Minnesota
Imaging Center
Rooms 23-35 Snyder Hall
1475 Gortner Avenue.
University of Minnesota,
St. Paul, MN 55108

Hours: 9:00-5:00 weekdays
Closed holidays

Adhering suspended samples onto coverslips

Solution: 0.1% polyethyleneimine

• 50% polyethyleneimine, aqueous (Sigma P-3143): 20.0 µL
  
• Q.S. with distilled H₂O: 10.0 mL
  

Procedure

1. Clean coverslips with 95% EtOH and allow to air dry
   
   
2. Add drop of 0.1% polyethyleneimine to coverslip and spread drop to cover surface of coverslip. Allow to incubate for 1-2 minutes. Do not allow to dry.
   
   
3. Briefly rinse coverslip with distilled water and allow to air dry (usually 10-20 minutes, use within 1 hour).
   
   
4. Add sample suspension to coverslip and allow to adhere for 10-15 minutes.
   
   
5. Wick off excess solution with filter paper and wait additional 2-3 minutes. DO NOT ALLOW TO AIR DRY!
   
   
6. Specimens are now mounted and ready to continue with additional processing.