Microwave Processing Workshop
May 17-19, 2006
Sponsored by Ted Pella, Inc. and the Imaging Center
A workshop focusing on new techniques in microwave specimen processing for light and electron microscopy.
In vivo labeling
Principles of microwave fixation for biological materials
Principles of immunolocalization for:
Confocal and fluorescence microscopy
Electron microscopy
In situ hybridization
As part of the Workshop we will have access to a state-of-the-art confocal system that will be provided by Nikon Instruments, Inc.
Nikon has recently introduced the C1si spectral imaging confocal system. The C1si has many patented design innovations that make acquiring spectral confocal images fast, accurate, and very sensitive. The unique C1si spectral detector has a 32 channel photomultiplier which allows the user to collect a spectral image of up to a 320nm spectral range in a single scan. The user can choose 2.5nm, 5nm, or 10nm resolution per channel. The C1si software allows easy and accurate un-mixing of closely overlapping emission spectra. The C1si is also a standard laser scanning confocal with a separate set of photomultipliers for standard confocal imaging, so the user can choose the confocal imaging mode that suits the experiment.
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