Perry Hackett, PhD
Research Techniques:
Methods involving recombinant DNA technology and analyzing gene expression in animals and their viruses. Methods for identifying integration sites of vectors in transgenic animals. Animal models include zebrafish, mice and dogs.
Research Interests:
The Hackett lab's main area of interest is using transposons as vectors for gene therapy as well as tagging and mapping genes in vertebrate chromosomes. For this, we use the Sleeping Beauty Transposon System, a system we created from extinct transposons that had been asleep for more than 10 million years. The lab works very closely with other labs in the Center for Genome Engineering including the McIvor, Largaespada, Somia and Voytas labs.
Selected Publications:
Fahrenkrug, S.C., Blake, A., Carlson, D.F., Doran, T., Van Eenennaam, A., Faber, D., Galli, C., Hackett, P.B., Li, Ning, Maga, E.A., Murray, J.D., Stotish, R., Sullivan, E., Taylor, J.F., Walton, Wheeler, M., Whitlaw, B., Glenn, B.P. (2010). Precision genetics for complex objectives in animal agriculture. J. Animal Sci. (in press)
Hackett, P.B., D.A. Largaespada and L.J.N. Cooper (2010) A transposon and transposase system for human application. Mol. Ther. 18: (in press)
Podetz-Pedersen, K., J.B. Bell, T.W.J. Steele, W.T. Scheir, L. Belur, R.S. McIvor and P.B. Hackett (2010). Gene expression in lung and liver after intravenous infusion of polyethylenimine complexes of Sleeping Beauty transposons. Hum. Gene Ther. 21: (in press).
Aronovich, E.L., J.B. Bell, S.A. Kahn, L.R. Belur, R. Gunther, B. Koniar, P.A. Schachern, J. Parker, C.S. Carlson, C.B. Whitley, R.S. McIvor, P. Gupta and P.B. Hackett (2009). Systemic correction of storage disease in MPS I NOD/SCID mice using the Sleeping Beauty transposon system. Mol. Ther. 17: 1136-1144.
Wallgard, E., M. Kalén, N. Asker, A. Nasevicius, L. Athley, A. Niemi, E. Billgren, J.D. Larson, S.A. Wadman, L. He, L. Karlsson, A.-K. Nilsson, T. Samuelsson, , J.J. Essner, P.B. Hackett and M. Hellström (2009). Combination of reverse- and chemical genetic screens reveals angiogenesis inhibitors and targets. Chem. Biol., 24: 432-441.
Singh, H., P.R. Manuari, S. Olivares, N. Dara, M.J. Dawson, H. Huls, P.B. Hackett, D,B. Kohn, E.J. Shipall, R. Champlin, and L.J.N. Cooper (2008). Redirecting specificity of T-cell populations for CD19 using the Sleeping Beauty System. Cancer Res. 68: 2961-2971.
Bell, J.B., K. Podetz-Pedersen, E.L. Aronovich, L. Belur, R.S. McIvor and P.B. Hackett (2007). Preferential delivery of the Sleeping Beauty transposon system to livers of mice by hydrodynamic injection. Nature Protocols 2 (12): 3153-3165.
Hackett, C.S., A.M. Geurts and P.B. Hackett (2007) Predicting DNA vector insertion sites: Implications for functional genomics and gene therapy. Genome Biol. 8 (Suppl 1): S12.
Aronovich, E.L., J.B. Bell, L.R. Belur, R. Gunther, B. Koniar, D.C. Erickson, P.A. Schachern, R.S. McIvor, C.B. Whitley and P.B. Hackett (2007). Sleeping Beauty transposon-mediated gene therapy of murine mucopolysaccharidosis. J. Gene Med.9: 403-415.
Hackett, P.B., S.C. Ekker, D.A. Largaespada and R.S. McIvor (2004). Sleeping Beauty transposon-mediated gene therapy for prolonged expression. In Huang, L., E. Wagner & M-C. Hung, eds. Non-Viral Vectors for Gene Therapy, 2nd Edition, Adv. Genet. 54: 187-229.
To view these and other publications visit http://www.ncbi.nlm.nih.gov/PubMed
search menu should say PubMed
type Hackett PB in the avaliable line
