Melissa Gardner, PhD

Research Techniques:

Computational Biology; Quantitative Fluorescence Microscopy; Single-molecule TIRF Microscopy, Genetics

Selected Publications:

Gardner, M.K., Zanic, M., Gell, C., Bormuth, V., and J. Howard, “Depolymerizing Kinesins Kip3 and MCAK Shape Cellular Microtubule Architecture by Differential Control of Catastrophe”, Cell (2011)

Gardner, M.K., Charlebois, B.D., Janosi, I.M., Howard, J., Hunt, A.J., and D.J. Odde, “Rapid Microtubule Self-assembly Kinetics”, Cell 146(4):1247-60. (2011)

Weaver, L.N., Ems-McClung, S.C.,…Gardner, M.K. and C.E. Walczak, “Kif18A Uses a Microtubule Binding Site in the Tail for Plus-End Localization and Spindle Length Regulation”, Current Biology 21(17):1500-6. (2011)

Demchouk, A.O., Gardner, M.K., D.J. Odde, “Microtubule Tip Tracking and Tip Structures at the Nanometer Scale Using Digital Fluorescence Microscopy”,Cellular and Molecular Bioengineering (2011)

Havens, K.A, Gardner, M.K., Kamieniecki, R.J., Dresser, M.E., and D.S. Dawson, “Slk19p of Saccharomyces cerevisiae Regulates Spindle Dynamics Through Two Independent Mechanisms, Genetics 186(4):1247-60. (2010)

Gardner, M.K., Sprague, B., Pearson, C., Cosgrove, B., Bicek, A., Bloom, K., Salmon, E.D., and D. J. Odde, “Model-Convolution: A computational approach to digital image interpretation”, Cellular and Molecular Bioengineering 3(2):163:170. (2010)

Gardner, M.K. and D.J. Odde, “Stochastic simulation and graphic visualization of mitotic processes”, METHODS 51(2):251-6. (2010)

Gardner, M.K., Bouck, D., Paliulis, L., Meehl, J., O’Toole, E., Haase, J., Joglekar, A., Winey, M., Salmon, E.D., Bloom, K., and D.J. Odde, “Chromosome congression by kinesin-5 motor-mediated disassembly of longer kinetochore microtubules”, Cell  135: 894-906 (2008).

Gardner, M.K., Odde, D.J. and K. Bloom, “Kinesin-8 molecular motors: putting the brakes on chromosome oscillations”, Trends in Cell Biology 18(7):307-10. (2008).

Gardner, M.K. and D.J. Odde, “Dam1 complexes go it alone on disassembling microtubules”, Nature Cell Biology, 10(4):379-81 (2008).

Gardner, M.K., Molk, J., Mythreye, K., Haase, J., Anderson, M., O’Toole, E., Joglekar, A., Winey, M., Odde, D., Salmon, E.D., and K. Bloom, “The microtubule-based motor Kar3 and plus-end binding protein Bim1 provide structural support for the anaphase spindle”, The Journal of Cell Biology, 180(1):91-100 (2008). 
**Paper reviewed in Current Biology Dispatch “Chromosome Segregation: organizing overlap at the midzone”, 18(7) (2008)
Gardner, M.K., Hunt, A.J., Goodson, H.V., and D.J. Odde, “Microtubule assembly dynamics: new insights at the nanoscale”, Current Opinion in Cell Biology, 20(1):64-70 (2008).

Schek, H.T*., Gardner, M.K.*, Cheng, J., Odde, D.J.**, and A.J. Hunt**, “ Microtubule assembly dynamics at the nanoscale”, Current Biology, 17(17):1445-55 (2007).  *Co-first Authors, **Co-directed Project
**Faculty of 1000 Recommended Reading

Gardner M.K., D. J. Odde, and K. Bloom, “Hypothesis testing via integrated computer modeling and digital fluorescence microscopy,” METHODS, 41(2): 232-7 (2007).

Gardner, M.K. and D.J. Odde, “Asymmetric division: motor persistence pays off”,Current Biology, 16(24), R1021-3 (2006).

 Gardner, M.K. and D.J. Odde, “Modeling of chromosome motility during mitosis”,Current Opinion in Cell Biology, 18(6), 639-47 (2006).

Shimogawa M, B Grackzyk, M.K. Gardner, S Francis, E White, M Ess, J Molk, C Ruse, S Niessen, J Yates, E Muller, K Bloom, D.J. Odde, and T.N. Davis, “Mps1 phosphorylation of the kinetochore component Dam1 couples kinetochores to microtubule plus-ends at metaphase,” Current Biology, 16(15), 1489-501 (2006).

Pearson, C.G*, M.K. Gardner*, L Paliulis, E.D. Salmon, D.J. Odde, and K. Bloom, “Measuring nanometer scale gradients in spindle microtubule dynamics using model convolution microscopy,” Molecular Biology of the Cell, 17(9), 4069-79, (2006), *Co-first Authors
Gardner, M.K., C. Pearson, B. Sprague, T. Zarzar, K. Bloom, E.D. Salmon, and D.J. Odde, “Tension-dependent regulation of microtubule dynamics at kinetochores can explain metaphase congression in yeast,” Molecular Biology of the Cell, 16, 3764-3775 (2005).
**Molecular Biology of the Cell Awarded  “Paper of the Year”  for this paper
**Faculty of 1000 Recommended Reading

Pearson, C.G., E. Yeh, M.K. Gardner, D.J. Odde, E.D. Salmon, and K. Bloom, “Stable kinetochore–microtubule attachment constrains centromere positioning in metaphase,” Current Biology, 14, 1962-1967 (2004).

To view these and other publications visit http://www.ncbi.nlm.nih.gov/PubMed
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type Gardner MK in the avaliable line