University of Minnesota
University of Minnesota
College of Biological Sciences
http://www.cbs.umn.edu/

Eric A. Hendrickson

Ph.D., Harvard Medical School, 1987
Professor, BMBB
Telephone: (612) 624-5988 Fax: (612) 625-2163

Division Head: Molecular Biology

Office: 6-102 MCB
Laboratory: 6-226 MCB
Lab Telephone: (612) 624-0935

Research Description

Our objective is to gain an understanding of the molecular and biochemical mechanisms of mammalian DNA double-strand break (DSB) repair. The importance of DNA DSB repair is underscored by the existence of several human cancer predisposition diseases, such as Nijmegen chromosome breakage syndrome, ataxia telangiectasia and Fanconi's anemia, which are caused by defects in DNA repair. The high cancer rate associated with these and other human diseases suggests that generalized DNA repair is also critical for many other important biological processes including chromosome integrity and stability. In particular, we have recently shown that DNA DSB repair is required for telomere maintenance and have demonstrated that defects in DNA DSB repair genes leads to telomere dysfunction and genomic instability. Our basic experimental model system uses gene targeting techniques in human cell lines in culture. In summary, the identification and characterization of the genes and protein factors involved in DSB repair will provide insight into the general mechanisms of DNA recombination and DNA repair and could have therapeutic significance for many types of immune disorders and cancers.


Recent Publications

Schumacher, A. J., Mohini, K. N., Kan, Y., Hendrickson, E. A., Stark, J. M., and Weller, S. K. The HSV-1 Exonuclease, UL12, Stimulates Recombination by a Single Strand Annealing Mechanism. (2012) PLoS Pathogens. in press.

Strande, N., Roberts, S. A., Oh, S., Hendrickson, E. A., and Ramsden, D. A. Specificity of the dRP/AP Lyase of Ku Promotes Nonhomologous End Joining (NHEJ) Fidelity at Damaged Ends.(2012) J. Biol. Chem., 287:13686-13693.

Fattah, F., Lee, E. H., Weisensel, N., Wang, Y., Lichter, N., and Hendrickson, E. A.  Ku regulates the non-homologous end joining pathway choice of double-strand breaks in human somatic cells.  (2010) PLoS Genetics, 6, e1000855.

Wang, Y., Ghosh, G. and Hendrickson, E. A.  Ku86 represses lethal telomere deletion events in human somatic cells.  (2009) Proc. Natl. Acad. Sci., USA, 106, 12430-12435.  {Profiled as a “Research Highlight” by Indiviglio, S. M. and Bertuch A.  (2009) Proc. Natl. Acad. Sci., USA, 106, 12217-1218.}

Ruis, B., Fattah, K. R., and Hendrickson, E. A. DNA-PKcs regulates proliferation, telomere length and genomic stability in human somatic cells. (2008) Mol. Cell. Biol., 28, 6182-6195.

Fattah, F., Lichter, N., Fattah, K. R., Oh, S., and Hendrickson, E. A. Ku70, an essential gene, modulates the frequency of rAAV-mediated gene targeting in human somatic cells. (2008) Proc. Natl. Acad. Sci., USA, 105, 8703-8708. Cited as a Research Highlight in Nature Struct. Mol. Biol., (2008) 15:699.

Fattah, K., Ruis, B., and Hendrickson, E. A. Mutations to Ku reveal differences in human somatic cell lines. (2008) DNA Repair, 7, 762-774.

Hendrickson, E. A. Gene targeting in human somatic cells. (2008) In: Sourcebook of Models for Biomedical Research. M. Conn, Ed., Humana Press, Totowa, NJ, pp. 509-525 (REVIEW).

Ghosh, G., Li, G., Myung, K., and Hendrickson, E. A. The lethality of Ku86 (XRCC5) loss-of-function mutations in human cells is independent of p53 (TP53). (2007) Rad. Res., 167, 66-79.

Hendrickson, E. A., Huffman, J. and Tainer, J. A. Structural aspects of Ku and the DNA dependent protein kinase complex. (2006) In: DNA Damage Recognition. W. Seide, Y. W. Kow and P. W. Doetsch, Eds., Taylor & Francis Group, Inc., Boca Raton, FL, pp. 629-684 (REVIEW).

Myung, K., Ghosh, G., Fattah, F. J., Li, G., Kim, H., Dutia, A., Pak, E., Smith, S. and Hendrickson, E. A. Regulation of telomere length and suppression of genomic instability in human somatic cells by Ku86. (2004) Mol. Cell. Biol., 24, 5050-5059

Myung, K., Ghosh, G., Fattah, F. J., Li, G., Kim, H., Dutia, A., Pak, E., Smith, S. and Hendrickson, E. A. Regulation of telomere length and suppression of genomic instability in human somatic cells by Ku86. (2004) Mol. Cell. Biol., 24:5050-5059.

Hendrickson, E. A. , Huffman, J. and Tainer, J. A. Structural aspects of Ku and the DNA dependent protein kinase complex. (2005) In: DNA Damage Recognition. W. Seide, Y. W. Kow and and P. W. Doetsch, Eds., Marcel-Dekker, Inc., New York, NY, in press. (Review)

Braastad, C. D., Han, Z., and Hendrickson, E. A. Constitutive DNaseI hypersensitivity of p53-regulated promoters. (2003) J. Biol. Chem., 278:8261-8268.

Hu, X., Han, Z., Wyche, J. H., and Hendrickson, E. A. Helix 6 of tBid is necessary but not sufficient for mitochondrial binding activity. (2003) Apoptosis, 8:277-289.

Han, Z., Wei, W., Dunaway, S., Darnowski, J. W., Calabresi, P., Sedivy, J., Hendrickson, E. A., Balan, K., Pantazis, P., and Wyche, J. H. Role of p21 in apoptosis and senesence of human colon cancer cells treated with camptotehcin. (2002) J. Biol. Chem., 277:17154-60. (PubMed citation).

Braastad, C. D., Leguia, M, and Hendrickson, E. A. Ku86 autoantigen related protein-1 transcription initiates from a CpG island and is induced by p53 through a nearby p53 response element. (2002) Nucl. Acids Res, 30, 1713-1724. (PubMed citation).

Li, G., Nelsen, C., and Hendrickson, E. A. Ku86 is essential in human somatic cells. (2002) Proc. Natl. Acad. Sci., USA, 99, 832-837. (PubMed citation).