Leap of faith (continued)
United they stand
To coax the yeast into forming multicellular clusters, the researchers applied evolutionary pressure in the form of conditions designed to improve the survival of cluster-forming yeast once they evolved. They centrifuged yeast cultures and saved only the bottom fluid, where any cell clusters would be the first to settle. Next, they transferred the fluid to a nutrient solution and grew the yeast for a day.
After 60 rounds of this procedure, their yeast had evolved to form 3-D clusters with branched "snowflake" profiles, where every cell in a single cluster was genetically identical. The snowflake yeast reproduce by splitting off multicellular juveniles that need a period of growth before maturing and producing their own offspring.
Evolution in action
The key step in the transition to a true multicellular life form is when natural selection and the rule of survival of the fittest begin to apply to clusters, says Ratcliff. At that point, clusters should start evolving as a unit. The team observed this with the snowflake yeast when they applied heavy evolutionary pressure for larger size and the clusters adapted. Specifically, they evolved to delay reproduction until they were bigger. (Reproduction, as shown in the accompanying time-lapse video, reduces a cluster's size.)
"Once adaptation is occurring in cluster-level traits, you're seeing multicellular evolution in action," says Travisano.
Another result of the transition was the discovery that some cells in the clusters had evolved to commit suicide. These dead cells act as break points within the snowflake yeast, allowing them to regulate the number and size of offspring they produce.
"An important hallmark of multicellularity is that you have many cells that give up selfish reproductive interests to benefit the whole organism," notes Ratcliff. "The fact that we see this evolving in our yeast after only 60 days is stunning."
The research team found that the sizes and suicide rates of clusters were genetic traits, due to naturally occurring gene mutations during the rounds of centrifugation and growth. Also, the clusters did not readily revert to their ancestors' solitary existence. Therefore, the team concluded, it appears that true evolution of multicellularity had occurred.
The researchers are continuing their yeast studies to discover how the transition to multicellularity affects the subsequent evolution of aging and, potentially, cancer.
And the transition isn't necessarily just a thing of the past.
"This could be going on today anywhere there's strong selection for larger size in microbes," Ratcliff says.
Other authors on the PNAS paper are R. Ford Denison and Mark Borrello, adjunct professor and associate professor, respectively, in the Department of Ecology, Evolution and Behavior.
– Deane Morrison
