This file created on 07/24/08 ====================================================== Strain: AA1 Species: Caenorhabditis elegans Genotype: daf-12(rh257) X. Description: daf-d. Strong heterochronic phenotypes in seam, somatic gonad, intestine. Class I allele. Occasional abnormal dauers under exhausted conditions. Mutagen: EMS Outcrossed: x Reference: CGC #3064 Antebi A;Culotti JG;Hedgecock EM daf-12 regulates developmental age and the dauer alternative in Caenorhabditis elegans. Development 125: 1191-1205 1998 Made by: Adam Antebi Received: 10/23/00 from Antebi A, Baylor College, Houston, TX -------------------- Strain: AA6 Species: Caenorhabditis elegans Genotype: daf-12(rh84) X. Description: daf-d. Strong heterochronic phenotypes in seam, somatic gonad, intestine. Class I allele. Mutagen: EMS Outcrossed: x2 Reference: CGC #3064 Antebi A;Culotti JG;Hedgecock EM daf-12 regulates developmental age and the dauer alternative in Caenorhabditis elegans. Development 125: 1191-1205 1998 Made by: Ed Hedgecock Received: 10/23/00 from Antebi A, Baylor College, Houston, TX -------------------- Strain: AA10 Species: Caenorhabditis elegans Genotype: daf-12(rh286) X. Description: Weak heterochronic phenotypes in seam, intestine, somatic gonad. Class V allele. Mutagen: EMS Outcrossed: x4 Reference: CGC #3064 Antebi A;Culotti JG;Hedgecock EM daf-12 regulates developmental age and the dauer alternative in Caenorhabditis elegans. Development 125: 1191-1205 1998 Made by: Adam Antebi Received: 10/23/00 from Antebi A, Baylor College, Houston, TX -------------------- Strain: AA18 Species: Caenorhabditis elegans Genotype: daf-12(rh61rh412) X. Description: daf-d. Weak heterochronic phenotypes in seam, somatic gonad and intestine. Class III allele. Mutagen: Spontaneous Outcrossed: x Reference: CGC #4181 Antebi A;Yeh WH;Tait D;Hedgecock EM;Riddle DL daf-12 encodes a nuclear receptor that regulates the dauer diapause and developmental age in C. elegans. Genes & Development 14: 1512-1527 2000 Made by: Adam Antebi Received: 11/03/00 from Antebi A, Baylor College, Houston, TX -------------------- Strain: AA34 Species: Caenorhabditis elegans Genotype: daf-12(rh61) X. Description: daf-d. Strong heterochronic phenotypes in seam, somatic gonad, intestine. Class I allele. Mutagen: EMS Outcrossed: x2 Reference: CGC #3064 Antebi A;Culotti JG;Hedgecock EM daf-12 regulates developmental age and the dauer alternative in Caenorhabditis elegans. Development 125: 1191-1205 1998 Made by: Ed Hedgecock Received: 10/23/00 from Antebi A, Baylor College, Houston, TX -------------------- Strain: AA82 Species: Caenorhabditis elegans Genotype: daf-12(rh284) X. Description: Gonadal lead cell Mig. Weak heterochronic phenotype in intestine. Weakly daf-c at 25C. Class V allele. Mutagen: EMS Outcrossed: x2 Reference: CGC #3064 Antebi A;Culotti JG;Hedgecock EM daf-12 regulates developmental age and the dauer alternative in Caenorhabditis elegans. Development 125: 1191-1205 1998 Made by: Adam Antebi Received: 10/23/00 from Antebi A, Baylor College, Houston, TX -------------------- Strain: AA83 Species: Caenorhabditis elegans Genotype: daf-12(rh62rh157) X. Description: daf-d. Strong heterochronic phenotypes in seam and intestine. Weak heterochronic phenotypes in somatic gonad. Class II allele. Mutagen: Spontaneous revertant Outcrossed: x3 Reference: CGC #3064 Antebi A;Culotti JG;Hedgecock EM daf-12 regulates developmental age and the dauer alternative in Caenorhabditis elegans. Development 125: 1191-1205 1998 Made by: Ed Hedgecock Received: 10/23/00 from Antebi A, Baylor College, Houston, TX -------------------- Strain: AA85 Species: Caenorhabditis elegans Genotype: daf-12(rh285) X. Description: Strong heterochronic phenotypes in seam, somatic gonad and intestine. Weakly daf-c at 15C. Class IV allele. Mutagen: EMS Outcrossed: x2 Reference: CGC #4181 Antebi A;Yeh WH;Tait D;Hedgecock EM;Riddle DL daf-12 encodes a nuclear receptor that regulates the dauer diapause and developmental age in C. elegans. Genes & Development 14: 1512-1527 2000 Made by: Adam Antevi Received: 11/03/00 from Antebi A, Baylor College, Houston, TX -------------------- Strain: AA86 Species: Caenorhabditis elegans Genotype: daf-12(rh61rh411) X. Description: Daf-d, weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele. Mutagen: Spontaneous revertant Outcrossed: x2 Reference: CGC #3064 Antebi A;Culotti JG;Hedgecock EM daf-12 regulates developmental age and the dauer alternative in Caenorhabditis elegans. Development 125: 1191-1205 1998 Made by: Adam Antebi Received: 10/23/00 from Antebi A, Baylor College, Houston, TX -------------------- Strain: AA87 Species: Caenorhabditis elegans Genotype: daf-12(rh273) X. Description: Daf-c, gonadal Mig, weak heterochronic phenotypes in intestine and seam. Class VI allele. Mutagen: EMS Outcrossed: x3 Reference: CGC #3064 Antebi A;Culotti JG;Hedgecock EM daf-12 regulates developmental age and the dauer alternative in Caenorhabditis elegans. Development 125: 1191-1205 1998 Made by: Adam Antebi Received: 10/23/00 from Antebi A, Baylor College, Houston, TX -------------------- Strain: AA88 Species: Caenorhabditis elegans Genotype: daf-12(rh193) X. Description: Strong heterochronic phenotypes in seam, somatic gonad and intestine. Heterochronic phenotypes less penetrant at 15C. Weakly daf-c at 25C. Class IV allele. Mutagen: Outcrossed: x3 Reference: CGC #4181 Antebi A;Yeh WH;Tait D;Hedgecock EM;Riddle DL daf-12 encodes a nuclear receptor that regulates the dauer diapause and developmental age in C. elegans. Genes & Development 14: 1512-1527 2000 Made by: Adam Antebi Received: 11/03/00 from Antebi A, Baylor College, Houston, TX -------------------- Strain: AA89 Species: Caenorhabditis elegans Genotype: daf-12(rh274) X. Description: daf-c. Gonald Mig. Weak heterochronic phenotypes in intestine. Class VI allele. Mutagen: EMS Outcrossed: x3 Reference: CGC #4181 Antebi A;Yeh WH;Tait D;Hedgecock EM;Riddle DL daf-12 encodes a nuclear receptor that regulates the dauer diapause and developmental age in C. elegans. Genes & Development 14: 1512-1527 2000 Made by: Adam Antebi Received: 11/03/00 from Antebi A, Baylor College, Houston, TX -------------------- Strain: AA107 Species: Caenorhabditis elegans Genotype: nhr-48(ok178) X. Description: ZK662.3 Homozygous. No obvious phenotype. Outer left primer sequence: TCTGAAGTTTGTGAGCCGTG. Outer right primer sequence: AGCGCCTAGATGAGCAACAT. Inner left primer sequence: TCCGTTGAATGCCATCTGTA. Inner right primer sequence: GGACGATGCACATGAGTTTG. Inner primer PCR product length: 3324 bp. Deletion size: 1956 bp. Mutagen: UV/TMP Outcrossed: x2 Reference: Made by: Adam Antebi Received: 08/19/03 from -------------------- Strain: AA120 Species: Caenorhabditis elegans Genotype: dhIs26. Description: dhIs26[daf-12A::GFP + lin-15(+)]. DAF-12::GFP localized primarily in nucleus, except during mitosis. Expressed widely in most cells including tissues modified for dauer formation or by stage from embryo to adult, but most elevated and widespread during L2. Mutagen: UV Outcrossed: x3 Reference: Made by: C. Kober-Eisermann Received: 03/23/07 from Antebi A, Baylor College, Houston, TX -------------------- Strain: AA278 Species: Caenorhabditis elegans Genotype: dhIs59. Description: dhIs59[Topo::daf-9::GFP + lin-15(+)]. Expressed in a ventral pair of bilateral neurons identified as the IL1Vs or URAVs in the anterior ganglia; perinuclear. By mid-L2, expression in the cytoplasm of the hypodermis, the syncitial epidermis, but absent from midline, epidermal seam cells. Levels peak around the L2 molt and diminish during L4. In some cases, transient expression seen in the L3 vulval blast cells. Also expressed within the hermaphrodite spermatheca starting in late L4 larvae and continuing eve in old adults. In males, expression in IL1V/URAVs and hypodermis but not somatic gonad. In dauer larvae, strong expression in IL1V/URAv and specifically extends into axonal but not dendritic processes. In post-dauer stages, expression in a pattern similar to reproductively growing animals, except expression is absent in the hypodermis. Grow at 20C. May still contain lin-15(n765) mutation in the background. Mutagen: UV Outcrossed: x3 Reference: Made by: C. Kober-Eisermann Received: 03/23/07 from Antebi A, Baylor College, Houston, TX -------------------- Strain: AA292 Species: Caenorhabditis elegans Genotype: daf-36(k114) V. Description: Mig on low cholesterol. Single daf-C at 27C, weak Mig. Strong expression in intestine at all stages. Grow at 20C. Mutagen: Outcrossed: x Reference: Made by: Veerle Rottiers Received: 03/23/07 from Antebi A, Baylor College, Houston, TX -------------------- Strain: AA408 Species: Caenorhabditis elegans Genotype: din-1(dh127) II. Description: daf-d, suppresses daf-12(rh61) daf-12(rh274) gonadal migration defects. Mutagen: EMS Outcrossed: x3 Reference: Made by: Andreas Ludewig Received: 01/28/08 from Antebi A, Baylor College, Houston, TX -------------------- Strain: AA411 Species: Caenorhabditis elegans Genotype: din-1(dh149) II. Description: daf-d, suppresses daf-12(rh61) daf-12(rh274) gonadal migration defects. Mutagen: EMS Outcrossed: x3 Reference: Made by: Andreas Ludewig Received: 01/28/08 from Antebi A, Baylor College, Houston, TX -------------------- Strain: AA426 Species: Caenorhabditis elegans Genotype: dre-1(dh99) V. Description: Precocious fusion of ceam cells one stage earlier prior to L3 molt; impenetrant gonadal migration defects; SynMig on daf-12 RNAi. Mutagen: EMS Outcrossed: x3 Reference: Made by: Nicole Fielenbach Received: 04/02/07 from Antebi A, Baylor College, Houston, TX -------------------- Strain: AA699 Species: Caenorhabditis elegans Genotype: din-1(hd36) II. Description: non-Daf. Temperature sensitive phenotypes: at 20C half of the animals are egg-laying defective with occasional mispositioned gonadal arms; at 25C, 18% arrest as embryos: those animals that hatch usually display variable morphology defects in body and pharynx; nearly all animals that live to adults were small, clear, and slightly uncoordinated, constipated, and virtually sterile. Maintain at 20C or below. Mutagen: Outcrossed: x Reference: Made by: Harald Hutter Received: 01/28/08 from Antebi A, Baylor College, Houston, TX -------------------- Strain: AA776 Species: Caenorhabditis elegans Genotype: cyp-44A1(ok216) II. Description: Mutagen: UV/TMP Outcrossed: x0 Reference: Made by: OMRF Knockout Group Received: 07/22/03 from Antebi A, Baylor College, Houston, TX -------------------- Strain: AA790 Species: Caenorhabditis elegans Genotype: lin-15B(n765) X; dhEx343. Description: dhEx343[lin-15(+) + L3781::din1pdin-1E::GFP]. din-1S::GFP is detected in hypodermis, seam, intestine, and somatic gonad including the distal tip cells. din-1S is also expressed in neurons, vulval precursors, body wall muscle, pharynx, and all tissues with heterochronic phenotypes or remodeled during dauer. Expression is first detected in a few nuclei by the comma stage of embryogenesis. By hatching, din-1S was widely expressed, albeit weakly. Overall expression in most tissues is detected at various levels into adult and in dauer larvae. Animals with the array are GFP+ and non-Muv. Animals which have lost the array are Muv and GFP-. Mutagen: Outcrossed: x Reference: Made by: Kerstin Neubert Received: 07/02/07 from Antebi A, Baylor College, Houston, TX -------------------- Strain: AB1 Species: Caenorhabditis elegans Genotype: C. elegans wild type (Tc1 pattern VII). Description: Reference WBG 10(2) 140-141 and WBG 8(2) 52. Caenorhabditis elegans wild isolate. Mutagen: Outcrossed: x Reference: CGC #2770 Hodgkin J;Doniach T Natural variation and copulatory plug formation in Caenorhabditis elegans. Genetics 146: 149-164 1997 Made by: Received: 09/01/84 from Bird A, CSIRO, Adelaide, Australia -------------------- Strain: AB2 Species: Caenorhabditis elegans Genotype: C. elegans wild type (Tc1 pattern VIII). Description: Reference WBG 10(2) 140-141 and WBG 8(2) 52. Caenorhabditis elegans wild isolate. Mutagen: Outcrossed: x Reference: CGC #2770 Hodgkin J;Doniach T Natural variation and copulatory plug formation in Caenorhabditis elegans. Genetics 146: 149-164 1997 Made by: Received: 09/01/84 from Bird A, CSIRO, Adelaide, Australia -------------------- Strain: AB3 Species: Caenorhabditis elegans Genotype: C. elegans wild type (Tc1 pattern VIII). Description: Reference WBG 10(2) 140-141 and WBG 8(2) 52. Caenorhabditis elegans wild isolate. Mutagen: Outcrossed: x Reference: CGC #2770 Hodgkin J;Doniach T Natural variation and copulatory plug formation in Caenorhabditis elegans. Genetics 146: 149-164 1997 Made by: Received: 09/01/84 from Bird A, CSIRO, Adelaide, Australia -------------------- Strain: AB4 Species: Caenorhabditis elegans Genotype: C. elegans wild type (Tc1 pattern VIII). Description: Reference WBG 10(2) 140-141 and WBG 8(2) 52. Caenorhabditis elegans wild isolate. Mutagen: Outcrossed: x Reference: CGC #2770 Hodgkin J;Doniach T Natural variation and copulatory plug formation in Caenorhabditis elegans. Genetics 146: 149-164 1997 Made by: Received: 09/01/84 from Bird A, CSIRO, Adelaide, Australia -------------------- Strain: AC68 Species: Caenorhabditis elegans Genotype: unc-29(e1072) aph-2(zu181)/unc-13(e1091) lin-11(n566) I. Description: Heterozygotes are WT and segregate WT, Unc Egls, and dead eggs. Mutagen: Tc1 Outcrossed: x10 Reference: CGC #4188 Goutte C;Hepler W;Mickey KM;Priess JR aph-2 encodes a novel extracellular protein required for GLP-1-mediated signaling. Development 127: 2481-2492 2000 Made by: Received: 11/09/00 from Goutte C, Amherst College, Amherst, MA -------------------- Strain: AC257 Species: Caenorhabditis elegans Genotype: ppk-3(n2668) X. Description: Growth retardation, enlarged vacuoles (late endosomes and lysosomes) in intestine, epidermis, coelomocytes and pharynx. 27% embryonic lethality and 8% post-embryonic lethality. Mutagen: EMS Outcrossed: x8 Reference: Made by: Andrew Chisholm Received: 04/25/07 from Labouesse M, IGBMC, Strasbourg, France -------------------- Strain: AD186 Species: Caenorhabditis elegans Genotype: egg-1(tm1071) III. Description: Temperature sensitive sterile. Fertility is <10% of WT at 25C. Grow at 20C. For phenotype, grow at 25C. Mutagen: UV/TMP Outcrossed: x>3 Reference: Made by: S. Mitani Received: 01/17/06 from Singson A, Waksman Institute, Piscataway, NJ -------------------- Strain: AD189 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; asIs2. Description: asIs2[unc-119(+) + ppie-1::GFP::egg-1]. Oocyte membranes are green. Mutagen: Microparticle bombardment Outcrossed: x Reference: Made by: Received: 03/27/06 from Singson A, Waksman Institute, Piscataway, NJ -------------------- Strain: AD200 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; asIs1. Description: asIs1[unc-119(+) + pie-1::GFP::egg-3]. Mutagen: Outcrossed: x3 Reference: Made by: Rika Maruyama Received: 03/20/08 from Singson A, Waksman Institute, Piscataway, NJ -------------------- Strain: AD226 Species: Caenorhabditis elegans Genotype: egg-3(tm1191)/mIn1[mIs14 dpy-10(e128)] II. Description: Heterozygotes are WT and GFP+. mIn1 homozygotes are Dpy and GFP+. egg-3 homozygotes are non-GFP. Mutagen: Outcrossed: x3 Reference: Made by: Japanese KO Consort. Received: 03/20/08 from Singson A, Waksman Institute, Piscataway, NJ -------------------- Strain: AD238 Species: Caenorhabditis elegans Genotype: asIs2. Description: asIs2[pie-1::mcherry::egg-3]. Mutagen: Outcrossed: x3 Reference: Made by: Rika Maruyama Received: 03/20/08 from Singson A, Waksman Institute, Piscataway, NJ -------------------- Strain: AD265 Species: Caenorhabditis elegans Genotype: nnIs2. Description: nnIs2[unc-119(+) Ppie-1::GFP::chs-1]. Mutagen: Outcrossed: x3 Reference: Made by: Rika Maruyama Received: 06/25/08 from Singson A, Waksman Institute, Piscataway, NJ -------------------- Strain: AE501 Species: Caenorhabditis elegans Genotype: nhr-8(ok186) IV. Description: Approx. 1.3 kb deletion - does not remove entire coding region, may not be null allele. No overt morphological or behavioral abnormalities. Homozygotes are 2-3X more sensitive to colchicine and chloroquine than are N2 animals. Mutagen: EMS Outcrossed: x4 Reference: CGC #4723 Lindblom TH;Pierce GJ;Sluder AE A C. elegans orphan nuclear receptor contributes to xenobiotic resistance. Current Biology 11: 864-868 2001 Made by: Moulder/Lindblom Received: 08/30/01 from Sluder A, Cambria Biosciences, Bedford, MA -------------------- Strain: AF1 Species: Caenorhabditis elegans Genotype: +/szT1[lon-2(e678)] I; dpy-8(e1321) unc-3(e151)/szT1 X. Description: Heterozygotes are WT and segregate WT, DpyUnc, dead eggs and Lon males. Maintain by picking WT. Mutagen: X-ray Outcrossed: x Reference: CGC #576 Fodor A;Deak P Isolation and phenocritical period-analysis of conditional and non-conditional developmental mutants in C. elegans. Acta Biologica Academiae Scientiarum Hungaricae 32: 229-239 1982 Made by: Fodor A Received: 05/01/81 from Fodor A, Hungarian Academy of Science, Szeged -------------------- Strain: AF5 Species: Rhabditis sp. Genotype: Wild isolate. Rhabditis sp. Description: Isolated in Towers Hill State Park in WI. Males are rare. No SDS resistant dauers. Animals are osmosensitive, can be dried competely and recover in water. Rhabditidae. Mutagen: Outcrossed: x Reference: Made by: Andras Fodor Received: 08/21/95 from Fodor A, Hungarian Academy of Science, Szeged -------------------- Strain: AF13(4) Species: Oscheius akosreti Genotype: Wild isolate. Oscheius akosreti. Description: Isolated in Memorial Park in Madison, WI. No hermaphrodites. Lots of SDS resistant dauers. They grow very slowly at 15C. Can survive between 15-28C. Mutagen: Outcrossed: x Reference: Made by: Andras Fodor Received: 08/21/95 from Fodor A, Hungarian Academy of Science, Szeged -------------------- Strain: AF16 Species: Caenorhabditis briggsae Genotype: C. briggsae wild type. Description: See WBG 7(1) 48. Isolated from soil in Ahmedabad, India. Previously called C. briggsae G16. Mutagen: Outcrossed: x Reference: CGC #692 Fodor A;Riddle DL;Nelson FK;Golden JW Comparison of a new wild-type Caenorhabditis briggsae with laboratory strains of C. briggsae and C. elegans. Nematologica 29: 203-217 1983 Made by: Andras Fodor Received: 02/01/82 from Fodor A, Hungarian Academy of Science, Szeged -------------------- Strain: AF23 Species: Mesorhabditis sp. Genotype: Wild isolate. Mesorhabditis sp. Description: Stock may be lost!! Growing stock at CGC lost, and didn't survive freezing well, so the freezer vials may not contain any live animals. Dark in color. Seems to grow better at 25C than 15C or 20C. Rhabditidae: Mesorhabditis sp. Mutagen: Outcrossed: x Reference: Made by: Andras Fodor Received: 08/21/95 from Fodor A, Hungarian Academy of Science, Szeged -------------------- Strain: AF25 Species: ? Genotype: Wild isolate. Not C. elegans. Description: Mutagen: Outcrossed: x Reference: Made by: Andras Fodor Received: 08/21/95 from Fodor A, Hungarian Academy of Science, Szeged -------------------- Strain: AF36 Species: Panagrolaimus rigidus Genotype: Wild isolate. Panagrolaimus rigidus. Description: Panagrolaimus rigidus (Schneider, 1886). Males and females. Animals are long. Mutagen: Outcrossed: x Reference: CGC #3766 Cunha A;Azevedo RBR;Emmons SW;Leroi AM Variable cell number in nematodes. Nature 402: 253 1999 Made by: Andras Fodor Received: 08/21/95 from Fodor A, Hungarian Academy of Science, Szeged -------------------- Strain: AF37 Species: Mesorhabditis sp. Genotype: Wild isolate. Mesorhabditis sp. Description: SDS resistant dauers. Rhabditida: Mesorhabditis sp. Mutagen: Outcrossed: x Reference: Made by: Andras Fodor Received: 08/21/95 from Fodor A, Hungarian Academy of Science, Szeged -------------------- Strain: AF40 Species: Panagrolaimus rigidus Genotype: Wild isolate. Panagrolaimus rigidus. Description: Panagrolaimidae: P. rigidus (Schneider, 1866). Long animals. Dig themselves into the agar. Mutagen: Outcrossed: x Reference: Made by: Andras Fodor Received: 08/21/95 from Fodor A, Hungarian Academy of Science, Szeged -------------------- Strain: AF72 Species: Mesorhabditis miotki Genotype: Wild isolate. Mesorhabditis miotki. Description: Rhabditidae: Mesorhabditis miotki, Sudhaus, 1978. Isolated in Pennsylvania. Mutagen: Outcrossed: x Reference: Made by: Andras Fodor Received: 08/21/95 from Fodor A, Hungarian Academy of Science, Szeged -------------------- Strain: AF78 Species: Mesorhabditis sp. Genotype: Wild isolate. Mesorhabditis sp. Description: Rhabditidae: Mesorhabditis sp. Isolated in Governor Dodge State Park in South Dakota. Dark in color. Mutagen: Outcrossed: x Reference: Made by: Andras Fodor Received: 08/21/95 from Fodor A, Hungarian Academy of Science, Szeged -------------------- Strain: AF271 Species: ? Genotype: Wild isolate. Not C. elegans. Description: Extremely long. Mutagen: Outcrossed: x Reference: Made by: Andras Fodor Received: 08/21/95 from Fodor A, Hungarian Academy of Science, Szeged -------------------- Strain: AF272 Species: ? Genotype: Wild isolate. Not C. elegans. Description: Dark in color. Mutagen: Outcrossed: x Reference: Made by: Andras Fodor Received: 08/21/95 from Fodor A, Hungarian Academy of Science, Szeged -------------------- Strain: AF6733 Species: ? Genotype: Wild isolate. Not C. elegans. Description: Isolated in Pennsylvania. SDS resistant dauers. males rare. Mutagen: Outcrossed: x Reference: Made by: Andras Fodor Received: 08/21/95 from Fodor A, Hungarian Academy of Science, Szeged -------------------- Strain: AF6840 Species: ? Genotype: Wild isolate. Not C. elegans. Description: Isolated in Pennsylvania. Males are rare. Mutagen: Outcrossed: x Reference: Made by: Andras Fodor Received: 08/21/95 from Fodor A, Hungarian Academy of Science, Szeged -------------------- Strain: AF7340 Species: ? Genotype: Wild isolate. Not C. elegans. Description: Isolated in Ohio. Mutagen: Outcrossed: x Reference: Made by: Andras Fodor Received: 08/21/95 from Fodor A, Hungarian Academy of Science, Szeged -------------------- Strain: AF8032 Species: ? Genotype: Wild isolate. Not C. elegans. Description: Isolated at Niagara Falls in Canada. SDS resistant dauers look like C. elegans predauers. Males are rare. Mutagen: Outcrossed: x Reference: Made by: Andras Fodor Received: 08/21/95 from Fodor A, Hungarian Academy of Science, Szeged -------------------- Strain: AF8130 Species: Pristionchus sp. Genotype: Wild isolate. Pristionchus sp. Description: Neodiplogasteridae: Pristionchus Iheritieri, Maupas, 1919. Isolated in Ontario, Canada. [9/98: Ralf Sommer has found this strain to be hermaphroditic. He had successful matings between AF8130 and PS312 Pristionchus pacificus, indicating that AF8130 is probably P. pacificus.] Mutagen: Outcrossed: x Reference: Made by: Andras Fodor Received: 08/21/95 from Fodor A, Hungarian Academy of Science, Szeged -------------------- Strain: AG50 Species: Caenorhabditis elegans Genotype: daf-7(e1372) dpy-1(e1) Description: Dpy. Temperature sensitive dauer constitutive. Crowds. 100% dauers at 25C. Leaky at 20C. Maintain at 15C. Mutagen: Outcrossed: x Reference: Made by: Andy Golden Received: 08/27/99 from Golden A, NIDDK/NIH, Bethesda, MD -------------------- Strain: AG150 Species: Caenorhabditis elegans Genotype: mat-2(ar104)/unc-4(e120) bli-1(e769) II. Description: Heterozygotes are WT and segregate WT, UncBli, and Steriles which have an everted vulva. ar104 previously called evl-22. Mutagen: Outcrossed: x Reference: CGC #1738 Seydoux G;Savage C;Greenwald I Isolation and characterization of mutations causing abnormal eversion of the vulva in Caenorhabditis elegans. Developmental Biology 157: 423-436 1993 Made by: Andy Golden Received: 05/30/01 from Golden A, NIDDK/NIH, Bethesda, MD -------------------- Strain: AG151 Species: Caenorhabditis elegans Genotype: cdc-25.1(nr2036)/dpy-5(e61) unc-13(e450) I. Description: Heterozygotes are WT and segregate WT, DpyUncs and adult steriles. Original deletion from Axys Pharmaceuticals. Mutagen: UV/TMP Outcrossed: x10 Reference: CGC #5294 Ashcroft N;Golden A CDC-25.1 regulates germline proliferation in Caenorhabditis elegans. Genesis 33: 1-7 2002 Made by: Ashcroft/Golden Received: 01/24/03 from Golden A, NIDDK/NIH, Bethesda, MD -------------------- Strain: AG152 Species: Caenorhabditis elegans Genotype: unc-85(e1414) bli-2(e768) dpy-10(e128) II. Description: Unc and Dpy. Not Blistered: dpy-10 suppresses the appearance of the blisters. Mutagen: Outcrossed: x Reference: Made by: Andy Golden Received: 06/17/05 from Golden A, NIDDK/NIH, Bethesda, MD -------------------- Strain: AG165 Species: Caenorhabditis elegans Genotype: san-1(av31) unc-13(e450) I. Description: Reduced brood size. Larval lethal, larval arrest. Steriles. Bursts at vulva. Can be maintained as a homozygote. Unc. Mutagen: EMS Outcrossed: x3 Reference: Made by: Ed Davis Received: 05/01/07 from Golden A, NIDDK/NIH, Bethesda, MD -------------------- Strain: AG166 Species: Caenorhabditis elegans Genotype: mdf-2(av16) unc-17(e245) IV. Description: Reduced brood size. Reduced hatching. Slow growth. Larval lethal. Larval arrest. Bursts at vulva. Suppresses the mat-3 one-cell arrest at 25C. Mutagen: EMS Outcrossed: x3 Reference: Made by: Received: 05/09/07 from Golden A, NIDDK/NIH, Bethesda, MD -------------------- Strain: AG168 Species: Caenorhabditis elegans Genotype: fzy-1(av15) unc-4(e120) II. Description: Unc. Gain-of-function allele of fzy-1. Mutagen: EMS Outcrossed: x Reference: Made by: Andy Golden Received: 06/06/07 from Golden A, NIDDK/NIH, Bethesda, MD -------------------- Strain: AG171 Species: Caenorhabditis elegans Genotype: mdf-1(av19) unc-42(e270) V. Description: Unc. Previously called AG164 by the CGC. Mutagen: EMS Outcrossed: x Reference: Made by: Andy Golden Received: 05/01/07 from Golden A, NIDDK/NIH, Bethesda, MD -------------------- Strain: AH12 Species: Caenorhabditis elegans Genotype: gap-1(ga133) X. Description: Null allele. Mutagen: Psoralen Outcrossed: x4 Reference: CGC #2916 Hajnal A;Whitfield CW;Kim SK Inhibition of Caenorhabditis elegans vulval induction by gap-1 and by let-23 receptor tyrosine kinase. Genes & Development 11: 2715-2728 1997 Made by: Alex Hajnal Received: 12/08/97 from Hajnal A, University of Zurich, Zurich, Switzerland -------------------- Strain: AH75 Species: Caenorhabditis elegans Genotype: apr-1(zh10) unc-29(e1072) I; zhEx11[apr-1(+); sur-5::GFP]. Description: Unc. Segregates dead eggs that have lost the rescuing array. Mutagen: EMS Outcrossed: x6 Reference: CGC #3991 Hoier EF;Mohler WA;Kim SK;Hajnal A The Caenorhabditis elegans APC-related gene apr-1 is required for epithelial cell migration and Hox gene expression. Genes & Development 14: 874-886 2000 Made by: Alex Hajnal Received: 07/24/00 from Hajnal A, University of Zurich, Zurich, Switzerland -------------------- Strain: AH102 Species: Caenorhabditis elegans Genotype: lip-1(zh15) IV. Description: Deletion allele which removes exons 2 to 6 of lip-1 (C05B10.1). Incompletely penetrant ovulation defect. Mutagen: EMS Outcrossed: x8 Reference: CGC #4542 Berset T;Hoier EF;Battu G;Canevascini S;Hajnal A Notch inhibition of Ras signaling through MAP kinase phosphatase LIP-1 during C. elegans vulval development. Science 291: 1055-1058 2001 Made by: Thomas Berset Received: 05/21/01 from Hajnal A, University of Zurich, Zurich, Switzerland -------------------- Strain: AH142 Species: Caenorhabditis elegans Genotype: zhIs4[pTB10] III. Description: lip-1::GFP transcriptional reporter. lip-1::GFP expression is upregulated in the secondary VPCs P5.p and P7.p of early L3 animals. Mutagen: Gamma Rays Outcrossed: x5 Reference: CGC #4542 Berset T;Hoier EF;Battu G;Canevascini S;Hajnal A Notch inhibition of Ras signaling through MAP kinase phosphatase LIP-1 during C. elegans vulval development. Science 291: 1055-1058 2001 Made by: Thomas Berset Received: 05/21/01 from Hajnal A, University of Zurich, Zurich, Switzerland -------------------- Strain: AH159 Species: Caenorhabditis elegans Genotype: sra-13(zh13) II. Description: sra-13(zh13) mutants display stronger chemotaxis to limiting concentrations of isoamylalcohol and diacetyl than WT animals. Deletion allele. 396 bp of 5' promoter sequence and all but the last exon are removed; probably a null allele. Mutagen: EMS Outcrossed: x8 Reference: CGC #5965 Battu G;Hoier EF;Hajnal A The C. elegans G-protein-coupled receptor SRA-13 inhibits RAS/MAPK signalling during olfaction and vulval development. Development 130: 2567-2577 2003 Made by: Gopal Battu Received: 12/16/03 from Hajnal A, University of Zurich, Zurich, Switzerland -------------------- Strain: AH205 Species: Caenorhabditis elegans Genotype: sdn-1(zh20) X. Description: Slightly Unc. Variably Egl. zh20 is a deletion in sdn-1. The sequence of the breakpoint is: TTTGCTTCACAC//zh20//GTCGACAGGCAG. Mutagen: EMS Outcrossed: x6 Reference: Made by: Erika Frohli Received: 10/26/05 from Hajnal A, University of Zurich, Zurich, Switzerland -------------------- Strain: AH286 Species: Caenorhabditis elegans Genotype: unc-4(e120) ect-2(zh8) II; gap-1(ga133) X. Description: Muv and Unc. Semi-dominant mutation in ect-2 (previously called let-21). Mutagen: EMS Outcrossed: x3 Reference: Made by: Erika Frohli Received: 12/27/05 from Hajnal A, University of Zurich, Zurich, Switzerland -------------------- Strain: AH346 Species: Caenorhabditis elegans Genotype: dep-1(zh34) unc-4(e120) II; lip-1(zh15) IV. Description: Pvl and weak Muv. Transformation of secondary to primary vulval cell fates. Mutagen: Outcrossed: x Reference: Made by: Thomas Berset Received: 12/27/05 from Hajnal A, University of Zurich, Zurich, Switzerland -------------------- Strain: AM1 Species: Caenorhabditis elegans Genotype: osr-1(rm1) I. Description: Osmotic stress resistant. Received new stock May 7, 2008. Mutagen: EMS Outcrossed: x4 Reference: CGC #6587 Solomon A;Bandhakavi S;Jabbar S;Shah R;Beitel GJ;Morimoto RI Caenorhabditis elegans OSR-1 regulates behavioral and physiological responses to hyperosmotic environments. Genetics 187: 161-170 2004 Made by: Aharon Solomon Received: 10/14/04 from Morimoto R, Northwestern University, Evanston, IL -------------------- Strain: AM134 Species: Caenorhabditis elegans Genotype: rmIs126. Description: rmIs126[P(unc-54) Q0::YFP]. Diffuse distribution of Q0::YFP throughout the body-wall muscle cells. Mutagen: Gamma Rays Outcrossed: x5 Reference: CGC #6510 Nollen EAA;Garcia SM;van Haaften G;Kim S;Chavez A;Morimoto RI;Plasterk RHA Genome-wide RNA interference screen identifies previously undescribed regulators of polyglutamine aggregation. Proceedings of the National Academy of Sciences USA 101: 6403-6408 2004 Made by: S. Garcia/A Chavez Received: 04/05/05 from Morimoto R, Northwestern University, Evanston, IL -------------------- Strain: AM138 Species: Caenorhabditis elegans Genotype: rmIs130 II. Description: rmIs130[P(unc-54) Q24::YFP]. Diffuse distribution of Q24::YFP throughout the body-wall muscle cells. Mutagen: Gamma Rays Outcrossed: x5 Reference: CGC #6510 Nollen EAA;Garcia SM;van Haaften G;Kim S;Chavez A;Morimoto RI;Plasterk RHA Genome-wide RNA interference screen identifies previously undescribed regulators of polyglutamine aggregation. Proceedings of the National Academy of Sciences USA 101: 6403-6408 2004 Made by: S. Garcia Received: 04/05/05 from Morimoto R, Northwestern University, Evanston, IL -------------------- Strain: AM140 Species: Caenorhabditis elegans Genotype: rmIs132 I. Description: rmIs132[P(unc-54) Q35::YFP]. AM140 animals show a Q35::YFP progressive transition from soluble to aggregated as they age. Mutagen: Gamma Rays Outcrossed: x5 Reference: CGC #6510 Nollen EAA;Garcia SM;van Haaften G;Kim S;Chavez A;Morimoto RI;Plasterk RHA Genome-wide RNA interference screen identifies previously undescribed regulators of polyglutamine aggregation. Proceedings of the National Academy of Sciences USA 101: 6403-6408 2004 Made by: S. Garcia/A Chavez Received: 04/05/05 from Morimoto R, Northwestern University, Evanston, IL -------------------- Strain: AM141 Species: Caenorhabditis elegans Genotype: rmIs133. Description: rmIs133[P(unc-54) Q40::YFP]. AM141 animals show a soluble Q40::YFP distribution in body wall muscle cells immediately after hatching. As these worms age the rapid formation of foci is observed. When they reach adulthood, AM141 animals show an entirely Q40::YFP aggregated phenotype. Mutagen: Gamma Rays Outcrossed: x5 Reference: CGC #6510 Nollen EAA;Garcia SM;van Haaften G;Kim S;Chavez A;Morimoto RI;Plasterk RHA Genome-wide RNA interference screen identifies previously undescribed regulators of polyglutamine aggregation. Proceedings of the National Academy of Sciences USA 101: 6403-6408 2004 Made by: S. Garcia/A Chavez Received: 04/05/05 from Morimoto R, Northwestern University, Evanston, IL -------------------- Strain: AN87 Species: Caenorhabditis elegans Genotype: sel-12(ty11) X. Description: Egl. Premature stop codon. Mutagen: EMS Outcrossed: x6 Reference: CGC #4859 Cinar HN;Sweet KL;Hosemann KE;Earley K;Newman AP The SEL-12 presenilin mediates induction of the Caenorhabditis elegans uterine pi cell fate. Developmental Biology 237: 173-182 2001 Made by: Nese Cinar Received: 12/04/01 from Newman A, Baylor College of Medicine, Houston, TX -------------------- Strain: AN170 Species: Caenorhabditis elegans Genotype: aff-1(ty4) unc-4(e120)/mnC1 dpy-10(e128) unc-52(e444) II. Description: Heterozygotes are WT and segregate WT, Unc-4 worms which are strong Egl, and paralyzed Dpy Uncs. Mutagen: EMS Outcrossed: x5 Reference: Made by: J Choi/A Newman Received: 02/19/08 from Baird S, Wright State University, Dayton, OH -------------------- Strain: AP36 Species: Caenorhabditis elegans Genotype: mep-1(ok421)/nT1[qIs51](IV;V). Description: Heterozygotes are WT and GFP+ and segregate 1/4 dead eggs (qIs51 homozygotes), 1/4 Mep hermaphrodites and dead eggs. Received new stock 12/02. Mutagen: UV/TMP Outcrossed: x5 Reference: CGC #5338 Belfiore M;Mathies LD;Pugnale P;Moulder G;Barstead R;Kimble J;Puoti A The MEP-1 zinc-finger protein acts with MOG DEAH box proteins to control gene expression via the fem-3 3'-untranslated region in Caenorhabditis elegans. RNA 8: 725-739 2002 Made by: A Puoti Received: 08/14/02 from Puoti A, University of Fribourg, Switzerland -------------------- Strain: AQ866 Species: Caenorhabditis elegans Genotype: ser-4(ok512) III. Description: Y22D7AR.13. Hyperactive 5HT induced egglaying. Homozygous. Outer Left Sequence: AATTATCGGATTTAGGGCCG. Outer Right Sequence: ATGGAACGGAGCATTATTCG. Inner Left Sequence: CAACACGCAACGAATGTACC. Inner Right Sequence: TGTGAAGTTTGGGAGGCTTT. Inner primer WT PCR product: 3126. Outcrossed 5 times by Stanley Shyn. URLs: http://aceserver.biotech.ubc.ca/cgi-bin/generic/allele?clas s=Allele;name=ok512, http://www.celeganskoconsortium.omrf.org/ Mutagen: UV/TMP Outcrossed: x5 Reference: Made by: Stanley Shyn Received: 02/24/03 from Schafer B, MRC-LMB, Cambridge, England -------------------- Strain: AR1 Species: Caenorhabditis elegans Genotype: hcp-6(mr17) I. Description: 15C: no phenotype. 26C: shifted as L4 or later results in 100% embryonic lethality; shifted before L4 results in Unc and Sterile animals. Intermediate phenotypes between 20-23C. Mutagen: EMS Outcrossed: x5 Reference: CGC #5322 Stear JH;Roth MB Characterization of HCP-6, a C. elegans protein required to prevent chromosome twisting and merotelic attachment. Genes & Development 16: 1498-1508 2002 Made by: Roth/Morrison/Stear Received: 11/04/02 from Roth M, Fred Hutchinson Cancer Research Center, Seattle, WA -------------------- Strain: AR2 Species: Caenorhabditis elegans Genotype: hcp-6(mr17) unc-73(e936) I. Description: Unc at all temperatures. For hcp-6: 15C: no phenotype. 26C: shifted as L4 or later results in 100% embryonic lethality; shifted before L4 results in Unc and Sterile animals. Intermediate phenotypes between 20-23C. Mutagen: Outcrossed: x Reference: CGC #5322 Stear JH;Roth MB Characterization of HCP-6, a C. elegans protein required to prevent chromosome twisting and merotelic attachment. Genes & Development 16: 1498-1508 2002 Made by: Jeff Stear Received: 11/04/02 from Roth M, Fred Hutchinson Cancer Research Center, Seattle, WA -------------------- Strain: AS270 Species: Caenorhabditis elegans Genotype: gly-12(id47) X. Description: Phenotypically WT. F3.2 See also CGC #5927. Mutagen: UV/TMP Outcrossed: x5 Reference: CGC #5572 Chen S;Tan J;Reinhold VN;Spence AM:Schachter H UDP-N-acetylglucosamine: alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I and UDP-N-acetylglucosamine: alpha-6-D-mannoside beta-1,2-N-acetylglucosaminyltransferase II in Caenorhabditis e Biochimica et Biophysica Acta 1573: 271-279 2002 Made by: Received: 03/12/03 from Schachter H, Hospital for Sick Children, Toronto, Ontario, Canada -------------------- Strain: AS271 Species: Caenorhabditis elegans Genotype: gly-14(id48) III. Description: Phenotypically WT. M8ns. See also CGC #5927. Mutagen: UV/TMP Outcrossed: x5 Reference: CGC #5572 Chen S;Tan J;Reinhold VN;Spence AM:Schachter H UDP-N-acetylglucosamine: alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I and UDP-N-acetylglucosamine: alpha-6-D-mannoside beta-1,2-N-acetylglucosaminyltransferase II in Caenorhabditis e Biochimica et Biophysica Acta 1573: 271-279 2002 Made by: Received: 03/12/03 from Schachter H, Hospital for Sick Children, Toronto, Ontario, Canada -------------------- Strain: AS338 Species: Caenorhabditis elegans Genotype: dpy-6(e14) gly-13(ok712) X. Description: Mutagen: UV/TMP Outcrossed: x5 Reference: CGC #6851 Zhu S;Hanneman A;Reinhold VN;Spence AM;Schachter H Caenorhabditis elegans triple null mutant lacking UDP-N-acetyl-D-glucosamine: alpha-3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I. Biochemical Journal 382: 995-1001 2004 Made by: Harry Schachter Received: 01/24/04 from Schachter H, Hospital for Sick Children, Toronto, Ontario, Canada -------------------- Strain: AT6 Species: Caenorhabditis elegans Genotype: srf-2(yj262)I. Description: WT. Antibody staining required to observe phenotype. Mutagen: EMS Outcrossed: x Reference: CGC #1280 Politz SM;Philipp M;Estevez M;O'Brien PJ;Chin KJ Genes that can be mutated to unmask hidden antigenic determinants in the cuticle of the nematode Caenorhabditis elegans. Proceedings of the National Academy of Sciences USA 87: 2901-2905 1990 Made by: Miguel Estevez Received: 06/18/90 from Politz S, Worcester Polytechnic Institute, Worcester, MA -------------------- Strain: AT10 Species: Caenorhabditis elegans Genotype: srf-3(yj10) IV. Description: WT. Antibody staining required to observe phenotype. Contains at least one extraneous mutation in the background (Eur. Worm Mtg 2006, Venuz et al.). Mutagen: Outcrossed: x Reference: CGC #1280 Politz SM;Philipp M;Estevez M;O'Brien PJ;Chin KJ Genes that can be mutated to unmask hidden antigenic determinants in the cuticle of the nematode Caenorhabditis elegans. Proceedings of the National Academy of Sciences USA 87: 2901-2905 1990 Made by: Received: 06/18/90 from Politz S, Worcester Polytechnic Institute, Worcester, MA -------------------- Strain: AU1 Species: Caenorhabditis elegans Genotype: sek-1(ag1) X. Description: Enhanced susceptibility to pathogens (esp). Egl-d. Nsy. Also called esp-2. Mutagen: EMS Outcrossed: x3 Reference: CGC #5370 Kim DH;Feinbaum R;Alloing G;Emerson FE;Garsin DA;Inoue H;Tanaka-Hino M;Hisamoto N;Matsumoto K;Tan MW;Ausubel FM A conserved p38 MAP kinase pathway in Caenorhabditis elegans innate immunity. Science 297: 623-626 2002 Made by: R. Feinbaum Received: 02/26/03 from Ausubel F, Massachusetts General Hospital, Boston, MA -------------------- Strain: AU3 Species: Caenorhabditis elegans Genotype: nsy-1(ag3) II. Description: Enhanced susceptibility to pathogens (esp). Nsy. Egl-d. Also called esp-8. Mutagen: EMS Outcrossed: x3 Reference: CGC #5370 Kim DH;Feinbaum R;Alloing G;Emerson FE;Garsin DA;Inoue H;Tanaka-Hino M;Hisamoto N;Matsumoto K;Tan MW;Ausubel FM A conserved p38 MAP kinase pathway in Caenorhabditis elegans innate immunity. Science 297: 623-626 2002 Made by: G. Alloing Received: 02/26/03 from Ausubel F, Massachusetts General Hospital, Boston, MA -------------------- Strain: AU37 Species: Caenorhabditis elegans Genotype: glp-4(bn2) I; sek-1(km4) X. Description: Temperature sensitive sterility. Maintain at 15C. Enhanced sensitivity to pathogens. Mutagen: Outcrossed: x Reference: Made by: Terence Moy Received: 04/16/08 from Ausubel F, Massachusetts General Hospital, Boston, MA -------------------- Strain: AU98 Species: Caenorhabditis elegans Genotype: inx-14(ag17) I. Description: Enhanced pathogen resistance. Mutagen: EMS Outcrossed: x5 Reference: Made by: Sachiko Miyata Received: 04/16/08 from Ausubel F, Massachusetts General Hospital, Boston, MA -------------------- Strain: AU147 Species: Caenorhabditis elegans Genotype: daf-16(mgDf47) I; glp-1(e2141) III. Description: Temperature sensitive sterility. Maintain at 15C. Mutagen: Outcrossed: x Reference: Made by: Sachiko Miyata Received: 04/16/08 from Ausubel F, Massachusetts General Hospital, Boston, MA -------------------- Strain: AU166 Species: Caenorhabditis elegans Genotype: daf-16(mgDf47) I; fog-2(q71) V. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x Reference: Made by: Sachiko Miyata Received: 05/19/08 from Ausubel F, Massachusetts General Hospital, Boston, MA -------------------- Strain: AV38 Species: Caenorhabditis elegans Genotype: mnDp66 (X;I); meDf2 X. Description: Produces 31% XO male self progeny; nondisjunction is correlated with a high frequency of achiasmate X chromosomes in oocyte nuclei, and a reduced frequency of X chromosome crossovers. meDf2 disrupts the function of the cis-acting X chromosome meiotic pairing center. meDf2/+ heterozygotes produce 4-6% XO progeny, so the presence of the Df can be followed in heterozygotes by following this weak Him phenotype. Mutagen: Gamma Rays Outcrossed: x2 Reference: CGC #1924 Villeneuve AM A cis-acting locus that promotes crossing over between X chromosomes in Caenorhabditis elegans. Genetics 136: 887-902 1994 Made by: Anne Villeneuve Received: 06/16/97 from Villeneuve A, Stanford University Medical School, Stanford, CA -------------------- Strain: AV39 Species: Caenorhabditis elegans Genotype: mnDp66 (X;I); meDf3 X. Description: Produces 32% XO male self progeny; nondisjunction is correlated with a high frequency of achiasmate X chromosomes in oocyte nuclei, and a reduced frequency of X chromosome crossovers. meDf3 disrupts the function of the cis-acting X chromosome meiotic pairing center. meDf3/+ heterozygotes produce 4-6% XO progeny, so the presence of the Df can be followed in heterozygotes by following this weak Him phenotype. Mutagen: Gamma Rays Outcrossed: x2 Reference: CGC #1924 Villeneuve AM A cis-acting locus that promotes crossing over between X chromosomes in Caenorhabditis elegans. Genetics 136: 887-902 1994 Made by: Anne Villeneuve Received: 06/16/97 from Villeneuve A, Stanford University Medical School, Stanford, CA -------------------- Strain: AV40 Species: Caenorhabditis elegans Genotype: mnDp66 (X;I); meDf4 X. Description: Produces 27% XO male self progeny; nondisjunction is correlated with a high frequency of achiasmate X chromosomes in oocyte nuclei, and a reduced frequency of X chromosome crossovers. meDf4 disrupts the function of the cis-acting X chromosome meiotic pairing center. meDf4/+ heterozygotes produce 4-6% XO progeny, so the presence of the Df can be followed in heterozygotes by following this weak Him phenotype. Mutagen: Gamma Rays Outcrossed: x2 Reference: CGC #1924 Villeneuve AM A cis-acting locus that promotes crossing over between X chromosomes in Caenorhabditis elegans. Genetics 136: 887-902 1994 Made by: Anne Villeneuve Received: 06/16/97 from Villeneuve A, Stanford University Medical School, Stanford, CA -------------------- Strain: AV41 Species: Caenorhabditis elegans Genotype: mnDp66 (X;I); meDf5 X. Description: Produces 32% XO male self-progeny; nondisjunction is correlated with a high frequency of achiasmate X chromosomes in oocyte nuclei, and a reduced frequency of X chromosome crossovers. meDf5 disrupts the function of the cis-acting X chromosome meiotic pairing center. meDf5/+ heterozygotes produce 4-6% XO progeny, so the presence of the Df can be followed in heterozygotes by following this weak Him phenotype. Mutagen: Gamma Rays Outcrossed: x2 Reference: CGC #1924 Villeneuve AM A cis-acting locus that promotes crossing over between X chromosomes in Caenorhabditis elegans. Genetics 136: 887-902 1994 Made by: Anne Villeneuve Received: 06/16/97 from Villeneuve A, Stanford University Medical School, Stanford, CA -------------------- Strain: AV51 Species: Caenorhabditis elegans Genotype: me8 X. Description: Homozygotes produce 10-15% XO males self progeny; nondisjuction is correlated with an increased frequency of achiasmate X chromosomes in oocyte nuclei, and an unaltered distribution of X chromosome crossovers. Heterozygotes produce 1-2% male self-progeny. Homozygotes (and XO hemizygotes) are slower growing than WT; reduced male mating efficiency. Disrupts the function ofthe cis-acting X chromosome meiotic pairing center. Molecular studies show that the me8 chromosome carries a terminal deletion that removes >70 kb from the left end of the X chromosome, including the endogenous telomere; further, a segment of chromosome V has been translocated to the left end of X, and a new telomere has been added de novo to the end of the translocated segment. Mutagen: EMS Outcrossed: x4 Reference: CGC #1924 Villeneuve AM A cis-acting locus that promotes crossing over between X chromosomes in Caenorhabditis elegans. Genetics 136: 887-902 1994 Made by: Anne Villeneuve Received: 06/16/97 from Villeneuve A, Stanford University Medical School, Stanford, CA -------------------- Strain: AV106 Species: Caenorhabditis elegans Genotype: spo-11(ok79) IV/nT1[unc-?(n754) let-?](IV;V). Description: Heterozygotes are Unc and segregate Uncs, dead eggs and WT. The WT (spo-11 homozygotes) produce about 200 fertilized eggs but only about 0.1 progeny that survive to adulthood. When mated to N2 males, spo-11 homozygotes will produce at least 5-10 cross progeny. Mutagen: Diepoxybutane Outcrossed: x8 Reference: CGC #3166 Dernburg AF;McDonald K;Moulder G;Barstead R;Dresser M;Villeneuve AM Meiotic recombination in C. elegans initiates by a conserved mechanism and is dispensable for homologous chromosome synapsis. Cell 94: 387-398 1998 Made by: Abby Dernburg Received: 10/30/98 from Villeneuve A, Stanford University Medical School, Stanford, CA -------------------- Strain: AV112 Species: Caenorhabditis elegans Genotype: mre-11(ok179) IV/nT1[unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc. mre-11 homozygotes produce about 200 fertilized eggs but only about 2-3% of these eggs survive to adulthood (this mutation cannot be maintained in a homozygous condition). Occasionally non-Unc progeny that do not demonstrate the mre-11(ok179) mutant phenotype arise when grown in large liquid cultures. mre-11 is the predict gene ZC302.1 Mutagen: Outcrossed: x Reference: CGC #4377 Kelly KO;Dernburg AF;Stanfield GM;Villeneuve AM Caenorhabditis elegans msh-5 is required for both normal and radiation-induced meiotic crossing-over but not for completion of meiosis. Genetics 156: 617-630 2000 Made by: Deletion Consortium Received: 12/04/00 from Villeneuve A, Stanford University Medical School, Stanford, CA -------------------- Strain: AV115 Species: Caenorhabditis elegans Genotype: msh-5(me23) IV/nT1[unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Uncs, dead eggs, and non-Uncs which give 97.9% dead eggs (of those that hatch, 42% are male). Mutagen: EMS Outcrossed: x Reference: CGC #4377 Kelly KO;Dernburg AF;Stanfield GM;Villeneuve AM Caenorhabditis elegans msh-5 is required for both normal and radiation-induced meiotic crossing-over but not for completion of meiosis. Genetics 156: 617-630 2000 Made by: Received: 03/06/02 from Villeneuve A, Stanford University Medical School, Stanford, CA -------------------- Strain: AV125 Species: Caenorhabditis elegans Genotype: spe-8(hc40) I; dpy-4(e1166) IV. Description: Can be maintained by chunking or setting up male/hermaphrodite crosses. Dpy. Mutagen: Outcrossed: x Reference: Made by: Gillian Stanfield Received: 10/30/06 from Stanfield G, University of Utah, Salt Lake City, UT -------------------- Strain: AV146 Species: Caenorhabditis elegans Genotype: chk-2(me64) rol-9(sc148)/unc-51(e369) rol-9(sc148) V. Description: Heterozygotes are Rollers and segregate Rollers, Unc Rollers, and Rollers which give 96-97% dead eggs (among the survivors are a high % of males). Mutagen: Outcrossed: x Reference: CGC #4767 MacQueen AJ;Villeneuve AM Nuclear reorganization and homologous chromosome pairing during meiotic prophase require C. elegans chk-2. Genes & Development 15: 1674-1687 2001 Made by: Amy MacQueen Received: 07/11/01 from Villeneuve A, Stanford University Medical School, Stanford, CA -------------------- Strain: AV157 Species: Caenorhabditis elegans Genotype: spo-11(me44)/nT1[unc-?(n754) let-? qIs50](IV;V). Description: Heterozygotes are Unc. spo-11(me44) homozygotes are viable and non-Unc. They produce more than 90% dead eggs and a large fraction of the survivors are males (strong Him phenotype). Cytologically they lack chiasmata in diakinesis-stage oocytes and lack RAD-51 foci. Maintain by picking Unc. Mutagen: EMS Outcrossed: x>2 Reference: Made by: Gregory Chin Received: 08/27/07 from Villeneuve A, Stanford University Medical School, Stanford, CA -------------------- Strain: AV158 Species: Caenorhabditis elegans Genotype: rad-50(ok197)/nT1[unc-?(n754) let-? qIs50](IV;V). Description: Heterozygotes are Unc and GFP+. rad-50(ok197) homozygotes are viable, non-Uncs. They produce 98.8% dead eggs and a large fraction of survivors are males (strong Him phenotype); cytologically they lack chiasmata in diakinesis-stage oocytes, but pairing and synapsis appear normal. Mutagen: Outcrossed: x4 Reference: Made by: Gregory Chin Received: 08/02/05 from Villeneuve A, Stanford University Medical School, Stanford, CA -------------------- Strain: AV267 Species: Caenorhabditis elegans Genotype: syp-3(me42)/hT2[bli-4(e937) let-?(q782) qIs48](I;III). Description: Heterozygotes are WT and GFP+. Segregate syp-3(me42) homozygotes that are non-GFP and lay mostly dead eggs; these mutants form abnormal synaptonemal complex formation during meiosis. Homozygous hT2[bli-4 let-? qIs48] animals are inviable. Mutagen: EMS Outcrossed: x4 Reference: Made by: J Engebrecht Received: 09/28/07 from Colaiacovo M, Harvard Medical School, Boston, MA -------------------- Strain: AV271 Species: Caenorhabditis elegans Genotype: him-3(me80)/nT1[unc-?(n754) let-? qIs50](IV;V). Description: Heterozygotes are Unc. him-3(me80) homozygotes are viable and non-Unc. They produce more than 85% dead eggs and a large fraction (11%) of the survivors are males (Him phenotype). Cytologically they exhibit a reduced level of HIM-3 loading and fewer stretches of SYP-1 than WT. In diakinesis-stage oocytes, they contain a mixture of bivalents and univalents. Maintain by picking Unc. Mutagen: EMS Outcrossed: x>2 Reference: Made by: Kentaro Nabeshima Received: 08/27/07 from Villeneuve A, Stanford University Medical School, Stanford, CA -------------------- Strain: AV276 Species: Caenorhabditis elegans Genotype: syp-2(ok307) V/nT1[unc-?(n754) let-?(m435)] (IV;V). Description: Heterozygotes are Unc. syp-2(ok307) homozygotes are viable, non-Unc. They produce 96% dead eggs and 44% males; cytologically they lack chiasmata in diakinesis-stage oocytes, exhibit persistent polarized nuclear organization during earlier meiotic prophase, lack synaptonemal complexes, and exhibit unstable pairing of homologous chromosomes. Mutagen: Outcrossed: x Reference: CGC #6086 Colaiacovo MP;MacQueen AJ;Martinez-Perez E;McDonald K;Adamo A;La Volpe A;Villeneuve AM Synaptonemal complex assembly in C. elegans is dispensable for loading strand-exchange proteins but critical for proper completion of recombination. Developmental Cell 5: 463-474 2003 Made by: Monica Colaiacovo Received: 09/16/04 from Villeneuve A, Stanford University Medical School, Stanford, CA -------------------- Strain: AV280 Species: Caenorhabditis elegans Genotype: unc-119(e2498) III; him-17(ok424) V; meIs5. Description: meIs5[unc-119(+) + him-17::GFP]. HIM-17::GFP is expressed in the germline. meIs5 not mapped. Mutagen: microparticle bombardment Outcrossed: x Reference: CGC #6740 Reddy KC;Villeneuve AM C. elegans HIM-17 links chromatin modification and competence for initiation of meiotic recombination. Cell 118: 439-452 2004 Made by: Kirthi Reddy Received: 09/16/04 from Villeneuve A, Stanford University Medical School, Stanford, CA -------------------- Strain: AV285 Species: Caenorhabditis elegans Genotype: swm-1(me66) him-5(e1490) V. Description: Sperm activation in virgin males. Poor sperm transfer. Mutagen: EMS Outcrossed: x6 Reference: Made by: Gillian Stanfield Received: 05/25/06 from Villeneuve A, Stanford University Medical School, Stanford, CA -------------------- Strain: AV307 Species: Caenorhabditis elegans Genotype: syp-1(me17) V/nT1[unc-?(n754) let-? qIs50] (IV;V). Description: Heterozygotes are Unc and GFP+. Segregates syp-1(me17) homozygotes which are viable, non-Unc, non-GFP; they produce 95% dead embryos and 38% males. Cytologically they lack chiasmata in diakinesis-stage oocytes, exhibit persistent polarized nuclear organization during earlier meiotic prophase, lack synaptonemal complexes, and exhibit unstable pairing of homologous chromosomes. qIs50 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter (F22B7.9) driving GFP in the intestine. Mutagen: EMS Outcrossed: x>2 Reference: CGC #5462 MacQueen AJ;Colaiacovo MP;McDonald K;Villeneuve AM Synapsis-dependent and -independent mechanisms stabilize homolog pairing during meiotic prophase in C. elegans. Genes & Development 16: 2428-2442 2002 Made by: Amy MacQueen Received: 09/25/03 from Villeneuve A, Stanford University Medical School, Stanford, CA -------------------- Strain: AV308 Species: Caenorhabditis elegans Genotype: him-14(it21)/mnC1 dpy-10(e128) unc-52(e444) II. Description: Heterozygotes are wild type and segregate wild type, DpyUncs, and him-14 homozygotes which are viable but produce >95% dead embryos and 45% males. Among these surviving progeny, cytologically they have 12 univalents in diakinesis-stage oocytes owing to a failure to form crossovers during meiosis. Mutagen: EMS Outcrossed: x Reference: CGC #3779 Zalevsky J;MacQueen AJ;Duffy JB;Kemphues KJ;Villeneuve AM Crossing over during Caenorhabditis elegans meiosis requires a conserved MutS-based pathway that is partially dispensable in budding yeast. Genetics 153: 1271-1283 1999 Made by: Received: 11/04/05 from Villeneuve A, Stanford University Medical School, Stanford, CA -------------------- Strain: AV311 Species: Caenorhabditis elegans Genotype: dpy-18(e364) unc-3(e151) meT7 (III;X;IV). Description: Dpy. Unc. meT7 is an end-to-end-to-end fusion of chromosomes III, X, and V. The right end of III is fused to the left end of X, and the right end of X is fused to the left end of IV. Constructed by crossing eT5 and mnT12. meT7 homozygotes produce 92% viable progeny. meT7 heterozygotes are Him and produce many dead eggs. Mutagen: Outcrossed: x>1 Reference: CGC #6115 Hillers KJ;Villeneuve AM Chromosome-wide control of meiotic crossing over in C. elegans. Current Biology 13: 1641-1647 2003 Made by: Kenneth Hillers Received: 12/05/03 from Villeneuve A, Stanford University Medical School, Stanford, CA -------------------- Strain: AV322 Species: Caenorhabditis elegans Genotype: swm-1(me87) him-5(e1490) V. Description: Sperm activation in virgin males. Very poor sperm transfer. Mutagen: EMS Outcrossed: x6 Reference: Made by: Gillian Stanfield Received: 05/25/06 from Villeneuve A, Stanford University Medical School, Stanford, CA -------------------- Strain: AX1295 Species: Caenorhabditis elegans Genotype: gcy-35(ok769) I. Description: Supresses aggregation and bordering phenotypes of npr-1(null) animals. Mutagen: UV/TMP Outcrossed: x5 Reference: CGC #6624 Cheung BHH;Arellano-Carbajal F;Rybicki I;de Bono M Soluble guanylate cyclases act in neurons exposed to the body fluid to promote C. elegans aggregation behavior. Current Biology 14: 1105-1111 2004 Made by: Mario de Bono Received: 12/07/04 from de Bono M, MRC-LMB, Cambridge, England -------------------- Strain: AX1296 Species: Caenorhabditis elegans Genotype: gcy-36(db42) X. Description: Supresses aggregation and bordering phenotypes of npr-1(null) animals. Mutagen: EMS Outcrossed: x8 Reference: CGC #6624 Cheung BHH;Arellano-Carbajal F;Rybicki I;de Bono M Soluble guanylate cyclases act in neurons exposed to the body fluid to promote C. elegans aggregation behavior. Current Biology 14: 1105-1111 2004 Made by: Mario de Bono Received: 12/07/04 from de Bono M, MRC-LMB, Cambridge, England -------------------- Strain: AX1297 Species: Caenorhabditis elegans Genotype: gcy-36(db66) X. Description: Supresses aggregation and bordering phenotypes of npr-1(null) animals. Mutagen: EMS Outcrossed: x8 Reference: CGC #6624 Cheung BHH;Arellano-Carbajal F;Rybicki I;de Bono M Soluble guanylate cyclases act in neurons exposed to the body fluid to promote C. elegans aggregation behavior. Current Biology 14: 1105-1111 2004 Made by: Mario de Bono Received: 12/07/04 from de Bono M, MRC-LMB, Cambridge, England -------------------- Strain: AX1305 Species: Caenorhabditis elegans Genotype: gcy-34(ok1012) V; npr-1(ad609) X. Description: Does not supress aggregation and bordering phenotypes of npr-1(null) animals. Mutagen: Outcrossed: x Reference: CGC #6244 Page AP;Winter AD Enzymes involved in the biogenesis of the nematode cuticle. Advances in Parasitology 53: 85-148 2003 Made by: Mario de Bono Received: 12/07/04 from de Bono M, MRC-LMB, Cambridge, England -------------------- Strain: AZ30 Species: Caenorhabditis elegans Genotype: sma-1(ru18) V. Description: Strong Sma phenotype. Null allele. Mutagen: UV/Psoralen Outcrossed: x3 Reference: CGC #3094 McKeown C;Praitis V;Austin J sma-1 encodes a B(H)-spectrin homolog required for Caenorhabditis elegans morphogenesis. Development 125: 2087-2098 1998 Made by: Vida Praitis Received: 05/07/01 from Austin J, University of Chicago, IL -------------------- Strain: AZ212 Species: Caenorhabditis elegans Genotype: unc-119(ed3) ruIs32[unc-119(+) pie-1::GFP::H2B] III. Description: Homozygous expression of GFP::H2B histone fusion in germline. pAZ132. Mutagen: microparticle bombardment Outcrossed: x0 Reference: CGC #4545 Praitis V;Casey E;Collar D;Austin J Creation of low-copy integrated transgenic lines in Caenorhabditis elegans. Genetics 157: 1217-1226 2001 Made by: Vida Praitis Received: 05/07/01 from Austin J, University of Chicago, IL -------------------- Strain: AZ217 Species: Caenorhabditis elegans Genotype: unc-119(ed3) ruIs37[unc-119(+) partial myo-2 promoter::GFP] III. Description: Expresses GFP in the pharynx. pAZ119. Mutagen: Microparticle bombardment Outcrossed: x0 Reference: CGC #4545 Praitis V;Casey E;Collar D;Austin J Creation of low-copy integrated transgenic lines in Caenorhabditis elegans. Genetics 157: 1217-1226 2001 Made by: Vida Praitis Received: 05/17/01 from Austin J, University of Chicago, IL -------------------- Strain: AZ218 Species: Caenorhabditis elegans Genotype: unc-119(ed3) ruIs38[unc-119(+) partial myo-2 promoter::GFP] III. Description: Expresses GFP in the pharynx. pAZ119. Mutagen: Microparticle bombardment Outcrossed: x0 Reference: CGC #4545 Praitis V;Casey E;Collar D;Austin J Creation of low-copy integrated transgenic lines in Caenorhabditis elegans. Genetics 157: 1217-1226 2001 Made by: Vida Praitis Received: 05/17/01 from Austin J, University of Chicago, IL -------------------- Strain: AZ235 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; ruIs48[unc-119(+) pie-1::tubulin::GFP fusion]. Description: Homozygous expression of GFP::tubulin fusion in germline and early embryos. Insertion unmapped. pAZ147. Mutagen: microparticle bombardment Outcrossed: x0 Reference: CGC #4545 Praitis V;Casey E;Collar D;Austin J Creation of low-copy integrated transgenic lines in Caenorhabditis elegans. Genetics 157: 1217-1226 2001 Made by: Vida Praitis Received: 05/07/01 from Austin J, University of Chicago, IL -------------------- Strain: AZ244 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; ruIs57[unc-119(+) pie-1::GFP::tubulin]. Description: Homozygous expression of GFP::tubulin fusion in germline and early embryos. Insertion unmapped. pAZ147. Mutagen: microparticle bombardment Outcrossed: x0 Reference: CGC #4545 Praitis V;Casey E;Collar D;Austin J Creation of low-copy integrated transgenic lines in Caenorhabditis elegans. Genetics 157: 1217-1226 2001 Made by: Vida Praitis Received: 05/07/01 from Austin J, University of Chicago, IL -------------------- Strain: BA1 Species: Caenorhabditis elegans Genotype: fer-1(hc1)I. Description: Temperature sensitive. M-MATING+LOW TEMP ONLY. Recessive. Fertilization abnormal. Mutagen: EMS Outcrossed: x Reference: CGC #393 Ward S;Miwa J Characterization of temperature-sensitive, fertilization-defective mutants of the nematode C. elegans. Genetics 88: 285-303 1978 Made by: Ward S Received: 03/01/80 from Herman B, University of Minnesota, Minneapolis, MN -------------------- Strain: BA2 Species: Caenorhabditis elegans Genotype: fer-2(hc2)III. Description: Temperature sensitive. Maintain at 15C. Some growth at 20C. Does not grow at 25C. Recessive. Mutagen: EMS Outcrossed: x Reference: CGC #495 Argon Y;Ward S C. elegans fertilization-defective mutants with abnormal sperm. Genetics 96: 413-433 1980 Made by: Ward S Received: 03/01/80 from Herman B, University of Minnesota, Minneapolis, MN -------------------- Strain: BA3 Species: Caenorhabditis elegans Genotype: fer-3(hc3)II. Description: Temperature sensitive. Fertilization abnormal. Recessive. Mutagen: EMS Outcrossed: x Reference: CGC #495 Argon Y;Ward S C. elegans fertilization-defective mutants with abnormal sperm. Genetics 96: 413-433 1980 Made by: Ward S Received: 03/01/80 from Herman B, University of Minnesota, Minneapolis, MN -------------------- Strain: BA4 Species: Caenorhabditis elegans Genotype: fer-4(hc4)V. Description: Fertilization abnormal. Recessive. Temperature sensitive. Maintain at 15C. Some growth at 20C and 25.4 C. Mutagen: EMS Outcrossed: x Reference: CGC #495 Argon Y;Ward S C. elegans fertilization-defective mutants with abnormal sperm. Genetics 96: 413-433 1980 Made by: Ward S Received: 03/01/80 from Herman B, University of Minnesota, Minneapolis, MN -------------------- Strain: BA6 Species: Caenorhabditis elegans Genotype: fer-6(hc6)I. Description: Fertilization abnormal. Temperature sensitive. Recessive. Mutagen: EMS Outcrossed: x Reference: CGC #1075 L'Hernault SW;Shakes DC;Ward S Developmental genetics of chromosome I spermatogenesis-defective mutants in the nematode Caenorhabditis elegans. Genetics 120: 435-452 1988 Made by: Ward S Received: 03/01/80 from Herman B, University of Minnesota, Minneapolis, MN -------------------- Strain: BA14 Species: Caenorhabditis elegans Genotype: fer-14(hc14)X. Description: Temperature sensitive. 8% of total oocytes produced are fertilized and give rise to larvae at 16C. Almost completely sterile at 25C. Mutagen: EMS Outcrossed: x2 Reference: CGC #558 Roberts TM;Ward S Membrane flow during nematode spermiogenesis. Journal of Cell Biology 92: 113-120 1982 Made by: Tom Roberts Received: 11/01/84 from Ward S, University of Arizona, Tucson -------------------- Strain: BA15 Species: Caenorhabditis elegans Genotype: fer-15(hc15)II. Description: Temperature sensitive. Maintain at 15C. Mutagen: EMS Outcrossed: x2 Reference: CGC #558 Roberts TM;Ward S Membrane flow during nematode spermiogenesis. Journal of Cell Biology 92: 113-120 1982 Made by: Tom Roberts Received: 11/01/84 from Ward S, University of Arizona, Tucson -------------------- Strain: BA17 Species: Caenorhabditis elegans Genotype: fem-1(hc17)IV. Description: Temperature sensitive. Recessive. Feminization. Mutagen: EMS Outcrossed: x Reference: CGC #252 Nelson GA;Lew KK;Ward S Intersex, a temperature-sensitive mutant of the nematode C. elegans. Developmental Biology 66: 386-409 1978 Made by: Nelson G Received: 03/01/80 from Herman B, University of Minnesota, Minneapolis, MN -------------------- Strain: BA23 Species: Caenorhabditis elegans Genotype: fer-6(hc23)I. Description: Temperature sensitive. Fertilization abnormal. Recessive. Mutagen: EMS Outcrossed: x Reference: CGC #492 Emmons SW;Rosenzweig B;Hirsh D Arrangement of repeated sequences in the DNA of the nematode C. elegans. Journal of Molecular Biology 144: 481-500 1980 Made by: Ward S Received: 03/01/80 from Herman B, University of Minnesota, Minneapolis, MN -------------------- Strain: BA24 Species: Caenorhabditis elegans Genotype: fer-1(hc24)I. Description: Fertilization abnormal. Recessive. Leaky ts. Mutagen: EMS Outcrossed: x Reference: CGC #393 Ward S;Miwa J Characterization of temperature-sensitive, fertilization-defective mutants of the nematode C. elegans. Genetics 88: 285-303 1978 Made by: Ward S Received: 03/01/80 from Herman B, University of Minnesota, Minneapolis, MN -------------------- Strain: BA34 Species: Caenorhabditis elegans Genotype: fer-7(hc34)I. Description: Fertilization abnormal. Recessive. Temperature sensitive. Maintain at 15C. Very Leaky-grows at 20C and also somewhat at 25.4C and 25.8C. Mutagen: EMS Outcrossed: x Reference: CGC #495 Argon Y;Ward S C. elegans fertilization-defective mutants with abnormal sperm. Genetics 96: 413-433 1980 Made by: Ward S Received: 03/01/80 from Herman B, University of Minnesota, Minneapolis, MN -------------------- Strain: BA521 Species: Caenorhabditis elegans Genotype: fer-1(hc1) unc-29(e1072) I. Description: Temperature sensitive. Maintain at 15C. Unc. Mutagen: Outcrossed: x Reference: CGC #393 Ward S;Miwa J Characterization of temperature-sensitive, fertilization-defective mutants of the nematode C. elegans. Genetics 88: 285-303 1978 Made by: Received: 02/01/86 from Ward S, University of Arizona, Tucson -------------------- Strain: BA522 Species: Caenorhabditis elegans Genotype: fer-1(hc13) unc-29(e1072)I. Description: Temperature sensitive. Maintain at 15C. Unc. Mutagen: EMS Outcrossed: x Reference: CGC #393 Ward S;Miwa J Characterization of temperature-sensitive, fertilization-defective mutants of the nematode C. elegans. Genetics 88: 285-303 1978 Made by: Received: 02/01/86 from Ward S, University of Arizona, Tucson -------------------- Strain: BA524 Species: Caenorhabditis elegans Genotype: fer-1(hc1)I; him-5(e1490)V. Description: Temperature sensitive. Maintain at 15C. Throws males. Mutagen: Outcrossed: x Reference: CGC #393 Ward S;Miwa J Characterization of temperature-sensitive, fertilization-defective mutants of the nematode C. elegans. Genetics 88: 285-303 1978 Made by: Y. Argon Received: 11/01/84 from Ward S, University of Arizona, Tucson -------------------- Strain: BA547 Species: Caenorhabditis elegans Genotype: fer-2(hc2)III; him-5(e1490)V. Description: Fertile at 16c and 20C. Sterile at 25C-Produces some defective embryos and 0-5 progeny. Throws males. Mutagen: Outcrossed: x Reference: CGC #529 Ward S;Argon Y;Nelson GA Sperm morphogenesis in wild-type and fertilization-defective mutants of C. elegans. Journal of Cell Biology 91: 26-44 1981 Made by: Received: 11/01/84 from Ward S, University of Arizona, Tucson -------------------- Strain: BA562 Species: Caenorhabditis elegans Genotype: fer-4(hc4) him-5(e1490)V. Description: Temperature sensitive. Maintain at 15C. Produces about 30% males. Mutagen: Outcrossed: x Reference: CGC #529 Ward S;Argon Y;Nelson GA Sperm morphogenesis in wild-type and fertilization-defective mutants of C. elegans. Journal of Cell Biology 91: 26-44 1981 Made by: Steve L'Hernault Received: 11/01/84 from Ward S, University of Arizona, Tucson -------------------- Strain: BA585 Species: Caenorhabditis elegans Genotype: spe-10(hc104) unc-76(e911)V. Description: Unc. Temperature sensitive, maintain at 16C. Average progeny: at 16C= 7 +/- 5; at 25C= 0.1 +/- .1. Mutagen: EMS Outcrossed: x Reference: CGC #1174 Shakes DC;Ward S Mutations that disrupt the morphogenesis and localization of a sperm-specific organelle in Caenorhabditis elegans. Developmental Biology 134: 307-316 1989 Made by: Steve L'Hernault Received: 02/03/93 from Ward S, University of Arizona, Tucson -------------------- Strain: BA590 Species: Caenorhabditis elegans Genotype: spe-10(hc104) dpy-11(e224)V. Description: Dpy. Temperature sensitive, maintain at 16C. Average progeny at 16C= 7 +/- 5. At 25C, average progeny = 0.1 =/- .1. Mutagen: EMS Outcrossed: x Reference: CGC #1174 Shakes DC;Ward S Mutations that disrupt the morphogenesis and localization of a sperm-specific organelle in Caenorhabditis elegans. Developmental Biology 134: 307-316 1989 Made by: Steve L'Hernault Received: 02/03/93 from Ward S, University of Arizona, Tucson -------------------- Strain: BA606 Species: Caenorhabditis elegans Genotype: spe-6(hc49) unc-25(e156)III; eDp6(III;f). Description: Animals with the Duplication are WT. Animals without the Duplication are Unc and Sterile. Mutagen: EMS Outcrossed: x10 Reference: CGC #1075 L'Hernault SW;Shakes DC;Ward S Developmental genetics of chromosome I spermatogenesis-defective mutants in the nematode Caenorhabditis elegans. Genetics 120: 435-452 1988 Made by: Steve L'Hernault Received: 02/03/93 from Ward S, University of Arizona, Tucson -------------------- Strain: BA609 Species: Caenorhabditis elegans Genotype: spe-6(hc49) vab-7(e1562)III; eDp6(III;f). Description: Animals with the Duplications are WT. Animals which have lost the Duplication are Sterile and have an abnormal tail. Mutagen: EMS Outcrossed: x10 Reference: CGC #1075 L'Hernault SW;Shakes DC;Ward S Developmental genetics of chromosome I spermatogenesis-defective mutants in the nematode Caenorhabditis elegans. Genetics 120: 435-452 1988 Made by: Steve L'Hernault Received: 02/03/93 from Ward S, University of Arizona, Tucson -------------------- Strain: BA671 Species: Caenorhabditis elegans Genotype: spe-9(hc88)I. Description: Self-sterile at 25C. Maintain at 15C. Mutagen: EMS Outcrossed: x>2 Reference: CGC #1075 L'Hernault SW;Shakes DC;Ward S Developmental genetics of chromosome I spermatogenesis-defective mutants in the nematode Caenorhabditis elegans. Genetics 120: 435-452 1988 Made by: Diane Shakes Received: 11/01/94 from L'Hernault S, Emory University, Atlanta, GA -------------------- Strain: BA676 Species: Caenorhabditis elegans Genotype: spe-6(hc92) unc-32(e189)III; eDp6(III;f). Description: Unc. Animals which have lost the duplications are Sterile (as well as Unc). Mutagen: EMS Outcrossed: x15 Reference: CGC #1075 L'Hernault SW;Shakes DC;Ward S Developmental genetics of chromosome I spermatogenesis-defective mutants in the nematode Caenorhabditis elegans. Genetics 120: 435-452 1988 Made by: Diane Shakes Received: 01/11/93 from Ward S, University of Arizona, Tucson -------------------- Strain: BA708 Species: Caenorhabditis elegans Genotype: spe-9(hc52)I. Description: Mutagen: EMS Outcrossed: x Reference: CGC #1075 L'Hernault SW;Shakes DC;Ward S Developmental genetics of chromosome I spermatogenesis-defective mutants in the nematode Caenorhabditis elegans. Genetics 120: 435-452 1988 Made by: Received: 06/15/88 from L'Hernault S, Emory University, Atlanta, GA -------------------- Strain: BA709 Species: Caenorhabditis elegans Genotype: fer-1(hc80) unc-29(e1072)/sDf6 I. Description: Heterozygotes are WT and segregate WT, Sterile Uncs and L1 lethals (sDf6/sDf6). hc80 is a nonconditional sterile. hc80 sperm have short pseudopods. Mutagen: EMS Outcrossed: x>3 Reference: CGC #1075 L'Hernault SW;Shakes DC;Ward S Developmental genetics of chromosome I spermatogenesis-defective mutants in the nematode Caenorhabditis elegans. Genetics 120: 435-452 1988 Made by: Steve L'Hernault Received: 10/17/94 from Ward S, University of Arizona, Tucson -------------------- Strain: BA714 Species: Caenorhabditis elegans Genotype: sDf5/spe-4(hc78)I. Description: Heterozygotes are WT and segregate WT, Steriles and dead eggs. Mutagen: EMS Outcrossed: x Reference: CGC #1075 L'Hernault SW;Shakes DC;Ward S Developmental genetics of chromosome I spermatogenesis-defective mutants in the nematode Caenorhabditis elegans. Genetics 120: 435-452 1988 Made by: Steve L'Hernault Received: 06/15/88 from L'Hernault S, Emory University, Atlanta, GA -------------------- Strain: BA717 Species: Caenorhabditis elegans Genotype: spe-11(hc90)I; sDp2(I;f). Description: WT. Animals which have lost the Dup are sterile. Maintain by picking fertile animals which throw steriles. Mutagen: EMS Outcrossed: x Reference: CGC #1075 L'Hernault SW;Shakes DC;Ward S Developmental genetics of chromosome I spermatogenesis-defective mutants in the nematode Caenorhabditis elegans. Genetics 120: 435-452 1988 Made by: Received: 06/15/88 from L'Hernault S, Emory University, Atlanta, GA -------------------- Strain: BA744 Species: Caenorhabditis elegans Genotype: spe-10(hc104)V. Description: Temperature sensitive, maintain at 15C. Average progeny at 16C is 7 +/- 5. Average progeny at 25C is 0.1 +/- .1. Mutagen: EMS Outcrossed: x Reference: CGC #1174 Shakes DC;Ward S Mutations that disrupt the morphogenesis and localization of a sperm-specific organelle in Caenorhabditis elegans. Developmental Biology 134: 307-316 1989 Made by: Diane Shakes Received: 02/03/93 from Ward S, University of Arizona, Tucson -------------------- Strain: BA759 Species: Caenorhabditis elegans Genotype: hcDf1 IV; eEx25. Description: Animals with eEx25 are WT. Animals which have lost eEx25 are Twitchers and Sterile (occasionally produce young). Mutagen: Spontaneous in TR466 Outcrossed: x Reference: CGC #1748 L'Hernault SW;Benian GM;Emmons RB Genetic and molecular characterization of the Caenorhabditis elegans spermatogenesis-defective gene spe-17. Genetics 134: 769-780 1993 Made by: Steve L'Hernault Received: 11/01/94 from L'Hernault S, Emory University, Atlanta, GA -------------------- Strain: BA763 Species: Caenorhabditis elegans Genotype: spe-5(hc93)I; sDp2(I;f). Description: Mutagen: EMS Outcrossed: x Reference: CGC #1075 L'Hernault SW;Shakes DC;Ward S Developmental genetics of chromosome I spermatogenesis-defective mutants in the nematode Caenorhabditis elegans. Genetics 120: 435-452 1988 Made by: Received: 06/15/88 from L'Hernault S, Emory University, Atlanta, GA -------------------- Strain: BA782 Species: Caenorhabditis elegans Genotype: spe-10(hc104) him-5(e1490)V. Description: Temperature sensitive, maintain at 16C. Segregates males. Average progeny at 16C is 7 +/- 5. Average progeny at 25C is 0.1 +/- .1. Mutagen: EMS Outcrossed: x Reference: CGC #1075 L'Hernault SW;Shakes DC;Ward S Developmental genetics of chromosome I spermatogenesis-defective mutants in the nematode Caenorhabditis elegans. Genetics 120: 435-452 1988 Made by: Diane Shakes. Received: 02/03/93 from Ward S, University of Arizona, Tucson -------------------- Strain: BA783 Species: Caenorhabditis elegans Genotype: spe-12(hc76)I. Description: Hermaphrodites produce nonfunctional sperm and are fertilization defective. Self-fertility is <1% at 16C or 25C. Males are fertile. Mutagen: EMS Outcrossed: x Reference: CGC #1075 L'Hernault SW;Shakes DC;Ward S Developmental genetics of chromosome I spermatogenesis-defective mutants in the nematode Caenorhabditis elegans. Genetics 120: 435-452 1988 Made by: Steve L'Hernault Received: 06/15/88 from L'Hernault S, Emory University, Atlanta, GA -------------------- Strain: BA784 Species: Caenorhabditis elegans Genotype: spe-8(hc50) I. Description: Male-hermaphrodite mating strain. Males are fertile, hermaphrodites are sterile. Mutagen: Steve L'Hernault Outcrossed: x Reference: CGC #1075 L'Hernault SW;Shakes DC;Ward S Developmental genetics of chromosome I spermatogenesis-defective mutants in the nematode Caenorhabditis elegans. Genetics 120: 435-452 1988 Made by: EMS Received: 01/05/01 from Ward S, University of Arizona, Tucson -------------------- Strain: BA785 Species: Caenorhabditis elegans Genotype: spe-8(hc40)I. Description: Hermaphrodites produce nonfunctional, nonmotile sperm with uniformly aberrant pseudopods. Male sperm normal. Self-fertility is <1% at 16C or 25C. Maintain by mating. Mutagen: EMS Outcrossed: x Reference: CGC #1075 L'Hernault SW;Shakes DC;Ward S Developmental genetics of chromosome I spermatogenesis-defective mutants in the nematode Caenorhabditis elegans. Genetics 120: 435-452 1988 Made by: Received: 06/15/88 from L'Hernault S, Emory University, Atlanta, GA -------------------- Strain: BA786 Species: Caenorhabditis elegans Genotype: spe-8(hc53) I. Description: Male-hermaphrodite mating strain. Males are fertile, hermaphrodites are sterile. Mutagen: Steve L'Hernault Outcrossed: x Reference: CGC #1075 L'Hernault SW;Shakes DC;Ward S Developmental genetics of chromosome I spermatogenesis-defective mutants in the nematode Caenorhabditis elegans. Genetics 120: 435-452 1988 Made by: EMS Received: 01/05/01 from Ward S, University of Arizona, Tucson -------------------- Strain: BA793 Species: Caenorhabditis elegans Genotype: spe-26(hc138) dpy-13(e184)IV. Description: Temperature sensitive. Fertile at 16C. Sterile at 25C. Partially fertile at 20C. Spermatogenesis arrests at teh spermatocyte stage. Mutagen: EMS Outcrossed: x15 Reference: CGC #1643 Van Voorhies WA Production of sperm reduces nematode life-span. Nature 360: 456-458 1992 Made by: Jacob Varkey Received: 02/03/93 from Ward S, University of Arizona, Tucson -------------------- Strain: BA794 Species: Caenorhabditis elegans Genotype: spe-13(hc137)I. Description: Temperature sensitive. Maintain at 15C. Mutagen: EMS Outcrossed: x Reference: CGC #1075 L'Hernault SW;Shakes DC;Ward S Developmental genetics of chromosome I spermatogenesis-defective mutants in the nematode Caenorhabditis elegans. Genetics 120: 435-452 1988 Made by: Received: 06/15/88 from L'Hernault S, Emory University, Atlanta, GA -------------------- Strain: BA811 Species: Caenorhabditis elegans Genotype: sDf5/spe-4(hc78) unc-15(e73)I. Description: Heterozygotes are WT and segregate WT, Sterile Unc and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Reference: CGC #1612 L'Hernault SW;Arduengo PM Mutation of a putative sperm membrane protein in Caenorhabditis elegans prevents sperm differentiation but not its associated meiotic divisions. Journal of Cell Biology 119: 55-68 1992 Made by: Steve L'Hernault Received: 06/15/88 from L'Hernault S, Emory University, Atlanta, GA -------------------- Strain: BA819 Species: Caenorhabditis elegans Genotype: spe-11(hc77)I. Description: Temperature sensitive sterile. Paternal effect embryonic lethal. Permissive temp is 20C. Restrictive temp is 25C. Mutagen: Outcrossed: x>4 Reference: CGC #1075 L'Hernault SW;Shakes DC;Ward S Developmental genetics of chromosome I spermatogenesis-defective mutants in the nematode Caenorhabditis elegans. Genetics 120: 435-452 1988 Made by: Diane Shakes Received: 10/17/94 from Ward S, University of Arizona, Tucson -------------------- Strain: BA821 Species: Caenorhabditis elegans Genotype: spe-26(hc138)IV. Description: Fertile at 15C; Sterile at 25C; Partially fertile at 20C. Spermatogenesis arrests at the spermatocyte stage. Increases lifespan of males and hermaphrodites. Mutagen: EMS Outcrossed: x15 Reference: CGC #1643 Van Voorhies WA Production of sperm reduces nematode life-span. Nature 360: 456-458 1992 Made by: Jacob Varkey Received: 01/11/93 from Ward S, University of Arizona, Tucson -------------------- Strain: BA824 Species: Caenorhabditis elegans Genotype: spe-26(hc139) dpy-20(e1282)IV. Description: Temperature sensitive. Partially fertile at 16C (very few progeny). Sterile at 20C and 25C. Weak Dpy at 15C. Spermatogenesis arrests at the spermatocyte stage. Mutagen: EMS Outcrossed: x15 Reference: Made by: Jacob Varkey Received: 02/03/93 from Ward S, University of Arizona, Tucson -------------------- Strain: BA825 Species: Caenorhabditis elegans Genotype: spe-26(hc140) dpy-20(e1282)/+ IV. Description: Heterozygotes are WT and segregate WT and DpySpe. The DpySpe are temperature sensitive. They are partially fertile at 16C (very few progeny), and sterile at 20C and 25C. Weak Dpy at 15C. Spermatogenesis arrests at the spermatocyte stage. Mutagen: EMS Outcrossed: x15 Reference: Made by: Jacob Varkey Received: 02/03/93 from Ward S, University of Arizona, Tucson -------------------- Strain: BA836 Species: Caenorhabditis elegans Genotype: spe-26(it112) unc-22(e66)IV. Description: Twitcher. Fertile at 15C; Sterile at 25C; Partially fertile at 20C. Spermatogenesis arrests at the spermatocyte stage. Mutagen: EMS Outcrossed: x10 Reference: CGC #1643 Van Voorhies WA Production of sperm reduces nematode life-span. Nature 360: 456-458 1992 Made by: Diane Shakes Received: 01/11/93 from Ward S, University of Arizona, Tucson -------------------- Strain: BA837 Species: Caenorhabditis elegans Genotype: spe-26(it112)IV. Description: Fertile at 15C; Sterile at 25C; Partially fertile at 20C. Spermatogenesis arrests at the spermatocyte stage. Mutagen: EMS Outcrossed: x10 Reference: CGC #1643 Van Voorhies WA Production of sperm reduces nematode life-span. Nature 360: 456-458 1992 Made by: Diane Shakes Received: 01/11/93 from Ward S, University of Arizona, Tucson -------------------- Strain: BA838 Species: Caenorhabditis elegans Genotype: spe-26(hc140)IV. Description: Temperature sensitive. Partially fertile at 16C (very few progeny). Sterile at 20C and 25C. Weak Dpy at 15C. Spermatogenesis arrests at the spermatocyte stage. Mutagen: EMS Outcrossed: x15 Reference: Made by: Jacob Varkey Received: 02/03/93 from Ward S, University of Arizona, Tucson -------------------- Strain: BA839 Species: Caenorhabditis elegans Genotype: spe-26(it118) unc-22(e66)IV. Description: Twitchers. Fertile at 15C; Sterile at 25C; Partially fertile at 20C. Spermatogenesis arrests at the spermatocyte stage. Mutagen: EMS Outcrossed: x10 Reference: CGC #1643 Van Voorhies WA Production of sperm reduces nematode life-span. Nature 360: 456-458 1992 Made by: Diane Shakes Received: 01/11/93 from Ward S, University of Arizona, Tucson -------------------- Strain: BA840 Species: Caenorhabditis elegans Genotype: spe-26(hc139)IV. Description: Temperature sensitive. Few progeny at 16C. Sterile at 20C and 25C. Spermatogenesis arrests at the spermatocyte stage. Mutagen: EMS Outcrossed: x15 Reference: Made by: Jacob Varkey Received: 02/03/93 from Ward S, University of Arizona, Tucson -------------------- Strain: BA843 Species: Caenorhabditis elegans Genotype: spe-26(it118)IV. Description: Temperature sensitive. Fertile at 16C. Sterile at 25C. Partially fertile at 20C. Spermatogenesis arrests at the spermatocyte stage. Mutagen: EMS Outcrossed: x10 Reference: Made by: Diane Shakes Received: 02/03/93 from Ward S, University of Arizona, Tucson -------------------- Strain: BA883 Species: Caenorhabditis elegans Genotype: spe-12(hc152) I. Description: Maintain by mating. Mutagen: Outcrossed: x Reference: CGC #3531 Nance J;Minniti AN;Sadler C;Ward S spe-12 encodes a sperm cell surface protein that promotes spermiogenesis in Caenorhabditis elegans. Genetics 152: 209-220 1999 Made by: Received: 01/05/01 from Ward S, University of Arizona, Tucson -------------------- Strain: BA900 Species: Caenorhabditis elegans Genotype: spe-27(it110)IV. Description: Male/hermaphrodite mating strain. Hermaphrodites self-sterile at all temps, males fertile. Spermatids activate "normally" in TEA. In pronase they produce spikes but never form pseudopods. Similar to spe-12 and spe-8. Mutagen: EMS Outcrossed: x Reference: CGC #2446 Minniti AN;Sadler C;Ward S Genetic and molecular analysis of spe-27, a gene required for spermiogenesis in Caenorhabditis elegans hermaphrodites. Genetics 143: 213-223 1996 Made by: Diane Shakes Received: 08/16/96 from Ward S, University of Arizona, Tucson -------------------- Strain: BA901 Species: Caenorhabditis elegans Genotype: spe-27(it110) dpy-20(e1282)IV. Description: Temperature sensitive Dpy. Male/hermaphrodite mating strain. Hermaphrodites self-sterile at all temps; males fertile. Spermatids arrest with spikes in pronase; form normal pseudopods in TEA. Mutagen: Outcrossed: x Reference: CGC #2446 Minniti AN;Sadler C;Ward S Genetic and molecular analysis of spe-27, a gene required for spermiogenesis in Caenorhabditis elegans hermaphrodites. Genetics 143: 213-223 1996 Made by: Received: 08/16/96 from Ward S, University of Arizona, Tucson -------------------- Strain: BA925 Species: Caenorhabditis elegans Genotype: spe-26(hc138) dpy-20(e1282)IV. Description: Temperature sensitive. Fertile at 16C. Sterile at 25C. Partially fertile at 20C. Weak Dpy at 15C. Spermatogenesis arrests at the spermatocyte stage. Mutagen: EMS Outcrossed: x15 Reference: Made by: Jacob Varkey Received: 02/03/93 from Ward S, University of Arizona, Tucson -------------------- Strain: BA947 Species: Caenorhabditis elegans Genotype: spe-27(hc161)IV. Description: Male/hermaphrodite mating strain. Hermaphrodites self-sterile at all temps; males fertile. Mutagen: EMS Outcrossed: x6 Reference: CGC #2446 Minniti AN;Sadler C;Ward S Genetic and molecular analysis of spe-27, a gene required for spermiogenesis in Caenorhabditis elegans hermaphrodites. Genetics 143: 213-223 1996 Made by: Alicia Minniti Received: 08/16/96 from Ward S, University of Arizona, Tucson -------------------- Strain: BA959 Species: Caenorhabditis elegans Genotype: spe-29(it127) dpy-20(e1282) IV. Description: Homozygous male/hermaphrodite line. Males are fertile. Hermaphrodites are sterile, but slightly leaky producing a few progeny (at 25C - ts not tested). In pronase, spermatids from males activate to form many long spikes, terminating at this stage. A very few (1-3 per worm) activate to form normal-looking, motile spermatozoons. Mutagen: Outcrossed: x Reference: CGC #4444 Nance J;Davis EB;Ward S spe-29 encodes a small predicted protein required for the initiation of sperm activation in Caenorhabditis elegans. Genetics 156: 1623-1633 2000 Made by: Received: 01/05/01 from Ward S, University of Arizona, Tucson -------------------- Strain: BA962 Species: Caenorhabditis elegans Genotype: spe-29(it127) IV. Description: Hermaphrodites are sterile; Males are fertile. Hermaphrodites lay oocytes (produce a few fertile eggs and many oocytes). Produce viable progeny when mated to males. Hermaphrodites produce 10X more self progeny at 20C (2.5/herm) than at either 16C or 25C. Mutagen: Outcrossed: x3 Reference: CGC #4444 Nance J;Davis EB;Ward S spe-29 encodes a small predicted protein required for the initiation of sperm activation in Caenorhabditis elegans. Genetics 156: 1623-1633 2000 Made by: Jeremy Nance Received: 01/05/01 from Ward S, University of Arizona, Tucson -------------------- Strain: BA963 Species: Caenorhabditis elegans Genotype: spe-27(it132)IV. Description: Temperature sensitive spe-27 allele. Hermaphrodites are sterile at 25C but produce 30-40 progeny/hermaphrodite at 16C. Males are fertile. Mutagen: EMS Outcrossed: x Reference: CGC #2446 Minniti AN;Sadler C;Ward S Genetic and molecular analysis of spe-27, a gene required for spermiogenesis in Caenorhabditis elegans hermaphrodites. Genetics 143: 213-223 1996 Made by: Received: 08/16/96 from Ward S, University of Arizona, Tucson -------------------- Strain: BA966 Species: Caenorhabditis elegans Genotype: spe-27(it132) unc-22(e66)IV. Description: Temperature sensitive spe-27 allele. Hermaphrodites sterile at 25C; hermaphrodites produce 30-40 progeny/hermaphrodite at 16C. Males cannot mate due to the unc-22 mutation. Maintain at 15C. Mutagen: Outcrossed: x Reference: CGC #2446 Minniti AN;Sadler C;Ward S Genetic and molecular analysis of spe-27, a gene required for spermiogenesis in Caenorhabditis elegans hermaphrodites. Genetics 143: 213-223 1996 Made by: Paul Muhlrad Received: 08/16/96 from Ward S, University of Arizona, Tucson -------------------- Strain: BA968 Species: Caenorhabditis elegans Genotype: spe-29(it127) unc-24(e138) IV. Description: Unc. Leaky sterile at 20C. Tighter at 16C and 25C. Mutagen: Outcrossed: x Reference: CGC #4444 Nance J;Davis EB;Ward S spe-29 encodes a small predicted protein required for the initiation of sperm activation in Caenorhabditis elegans. Genetics 156: 1623-1633 2000 Made by: Jeremy Nance Received: 01/05/01 from Ward S, University of Arizona, Tucson -------------------- Strain: BA969 Species: Caenorhabditis elegans Genotype: spe-6(hc163) III; spe-27(it132) dpy-20(e1282) IV. Description: Dpy. spe-6(hc163) is a recessive suppressor of spe-27(it132). spe-6(hc163) also suppresses spe-8, spe-12, spe-29 and other spe-27 alleles. Causes precocious spermatid activation. Fertile between 15-25C. Mutagen: EMS (hc163) Outcrossed: x5 Reference: CGC #5276 Muhlrad PJ;Ward S Spermiogenesis initiation in Caenorhabditis elegans involves a casein kinase 1 encoded by the spe-6 gene. Genetics 161: 143-155 2002 Made by: Paul Muhlrad Received: 07/29/03 from Ward S, University of Arizona, Tucson -------------------- Strain: BA970 Species: Caenorhabditis elegans Genotype: spe-6(hc163) III; spe-27(it132) unc-22(e66) IV. Description: Twitcher Unc. spe-6(hc163) is a recessive suppressor of spe-27(it132). spe-6(hc163) also suppresses spe-8, spe-12, spe-29 and other spe-27 alleles. Causes precocious spermatid activation. Fertile between 15-25C. Mutagen: EMS (hc163) Outcrossed: x4 Reference: CGC #5276 Muhlrad PJ;Ward S Spermiogenesis initiation in Caenorhabditis elegans involves a casein kinase 1 encoded by the spe-6 gene. Genetics 161: 143-155 2002 Made by: Paul Muhlrad Received: 07/29/03 from Ward S, University of Arizona, Tucson -------------------- Strain: BA971 Species: Caenorhabditis elegans Genotype: spe-27(it132) dpy-20(e1282)IV. Description: Temperature sensitive spe-27 allele. Leaky sterile at 20C. Males at 20C produce spermatids that form spikes in pronase. Males are fertile. Temperature sensitive dpy-20 allele. Maintain at 15C. Mutagen: Outcrossed: x Reference: CGC #2446 Minniti AN;Sadler C;Ward S Genetic and molecular analysis of spe-27, a gene required for spermiogenesis in Caenorhabditis elegans hermaphrodites. Genetics 143: 213-223 1996 Made by: Received: 08/16/96 from Ward S, University of Arizona, Tucson -------------------- Strain: BA975 Species: Caenorhabditis elegans Genotype: spe-6(hc163) III; spe-29(it127) dpy-20(e1282) IV. Description: spe-6(hc163) suppresses the self-sterility of spe-29 in this strain. Self-sterile at 25C. Mutagen: Outcrossed: x Reference: CGC #5276 Muhlrad PJ;Ward S Spermiogenesis initiation in Caenorhabditis elegans involves a casein kinase 1 encoded by the spe-6 gene. Genetics 161: 143-155 2002 Made by: Paul Muhlrad Received: 02/28/05 from Ward S, University of Arizona, Tucson -------------------- Strain: BA979 Species: Caenorhabditis elegans Genotype: spe-6(hc163) III; spe-12(hc76) dpy-5(e61) IV. Description: spe-6(hc163) suppresses hermaphrodite self-sterility of hc76. Dpy. Mutagen: Outcrossed: x Reference: CGC #5276 Muhlrad PJ;Ward S Spermiogenesis initiation in Caenorhabditis elegans involves a casein kinase 1 encoded by the spe-6 gene. Genetics 161: 143-155 2002 Made by: Paul Muhlrad Received: 02/28/05 from Ward S, University of Arizona, Tucson -------------------- Strain: BA984 Species: Caenorhabditis elegans Genotype: spe-6(hc163) dpy-18(e364) III. Description: Dpy. spe-6(hc163) suppresses self-sterility of spe-8, spe-12, spe-27, and spe-29 mutants. Causes precocious spermatide activation. Fertile between 15-25C. Mutagen: EMS (hc163) Outcrossed: x Reference: CGC #5276 Muhlrad PJ;Ward S Spermiogenesis initiation in Caenorhabditis elegans involves a casein kinase 1 encoded by the spe-6 gene. Genetics 161: 143-155 2002 Made by: Paul Muhlrad Received: 07/29/03 from Ward S, University of Arizona, Tucson -------------------- Strain: BA989 Species: Caenorhabditis elegans Genotype: spe-6(hc163) III; spe-27(it132) IV. Description: spe-6(hc163) suppresses the ts self-sterile phenotype of spe-27(it132). Also suppresses spe-8, spe-12, and spe-29. Causes precocious spermatid activation. Lays eggs and oocytes. Males are weakly fertile. Fertile between 15-25C. Mutagen: EMS (hc163) Outcrossed: x Reference: CGC #5276 Muhlrad PJ;Ward S Spermiogenesis initiation in Caenorhabditis elegans involves a casein kinase 1 encoded by the spe-6 gene. Genetics 161: 143-155 2002 Made by: Paul Muhlrad Received: 07/29/03 from Ward S, University of Arizona, Tucson -------------------- Strain: BA1013 Species: Caenorhabditis elegans Genotype: spe-6(hc49) vab-7(e1562)/qC1 dpy-19(e1259) glp-1(q339) III; spe-27(it132) IV. Description: Male/hermaphrodite line. Maintain at 15C to insure maintenance of male/hermaphrodite line. Mutagen: Outcrossed: x Reference: CGC #5276 Muhlrad PJ;Ward S Spermiogenesis initiation in Caenorhabditis elegans involves a casein kinase 1 encoded by the spe-6 gene. Genetics 161: 143-155 2002 Made by: Paul Muhlrad Received: 02/28/05 from Ward S, University of Arizona, Tucson -------------------- Strain: BA1044 Species: Caenorhabditis elegans Genotype: spe-27(it132) spe-29(it127) IV; him-5(e1490) V. Description: Non-conditional hermaphrodite sterile. Males fertile. Self-progeny produced after mating. Mutagen: Outcrossed: x Reference: CGC #4444 Nance J;Davis EB;Ward S spe-29 encodes a small predicted protein required for the initiation of sperm activation in Caenorhabditis elegans. Genetics 156: 1623-1633 2000 Made by: Jeremy Nance Received: 01/05/01 from Ward S, University of Arizona, Tucson -------------------- Strain: BA1051 Species: Caenorhabditis elegans Genotype: spe-12(hc76) I; spe-29(it127) IV; him-5(e1490) V. Description: Self-sterile hermaphrodites; fertile males. Mated hermaphrodites produce self and outcross progeny. Self progeny are 33% males. Mutagen: Outcrossed: x Reference: CGC #4444 Nance J;Davis EB;Ward S spe-29 encodes a small predicted protein required for the initiation of sperm activation in Caenorhabditis elegans. Genetics 156: 1623-1633 2000 Made by: Jeremy Nance Received: 01/05/01 from Ward S, University of Arizona, Tucson -------------------- Strain: BA1061 Species: Caenorhabditis elegans Genotype: dpy-18(e364) spe-6(hc49) ale-1(mc14)/qC1 dpy-19(e1259) glp-1(q339) III. Description: Heterozygotes are WT and segregate WT, Sterile Dpys (Dpy is temperature sensitive), and dead eggs. ale-1 is a recessive embryonic lethal. Mutagen: Outcrossed: x Reference: CGC #5276 Muhlrad PJ;Ward S Spermiogenesis initiation in Caenorhabditis elegans involves a casein kinase 1 encoded by the spe-6 gene. Genetics 161: 143-155 2002 Made by: Paul Muhlrad Received: 02/28/05 from Ward S, University of Arizona, Tucson -------------------- Strain: BA1069 Species: Caenorhabditis elegans Genotype: F26F4.8(hc180) III. Description: Deletion allele of F26F4.8. Homologous to Zn finger of Drosophila ovo transcription factor. Developmental defects in hind gut, germline and vulva. HES primers 106/107 outer and HES 108/109 inner. Mutagen: UV/TMP Outcrossed: x6 Reference: Made by: H. Smith Received: 10/15/01 from Ward S, University of Arizona, Tucson -------------------- Strain: BA1070 Species: Caenorhabditis elegans Genotype: cdh-5(hc181) IV. Description: Deletion allele of F08B4.2. Homologous to Drosophila fat cadherin. Primers HES 114/115 outer and HES 116/117 inner. Mutagen: UV/TMP Outcrossed: x2 Reference: Made by: H. Smith Received: 10/15/01 from Ward S, University of Arizona, Tucson -------------------- Strain: BA1073 Species: Caenorhabditis elegans Genotype: dyf-5(hc183) I. Description: Deletion allele of M04C9.5 (mak-kinase). No visible phenotype in hermaphrodites. Primers HES 186/187 outer and HES 188/189 inner. Mutagen: Outcrossed: x0 Reference: Made by: H. Smith Received: 10/15/01 from Ward S, University of Arizona, Tucson -------------------- Strain: BA1083 Species: Caenorhabditis elegans Genotype: F43G6.6(hc184) II. Description: Deletion allele of F43G6.6. No obvious phenotype in hermaphrodites. HES primers 162/163 outer and 164/165 inner. Mutagen: UV/TMP Outcrossed: x0 Reference: Made by: H. Smith Received: 10/15/01 from Ward S, University of Arizona, Tucson -------------------- Strain: BA1084 Species: Caenorhabditis elegans Genotype: F09C12.2(hc185) II. Description: Deletion allele of F09C12.2. No obvious phenotype in hermaphrodites. HES primers 122/123 outer and HES 124/125 inner. Mutagen: UV/TMP Outcrossed: x0 Reference: Made by: H. Smith Received: 10/15/01 from Ward S, University of Arizona, Tucson -------------------- Strain: BA1090 Species: Caenorhabditis elegans Genotype: cav-2(hc191) V. Description: No visible phenotype in hermaphrodites. Deletion allele of C56A3.7. Mutagen: TMP Outcrossed: x6 Reference: Made by: HES Received: 07/26/01 from Ward S, University of Arizona, Tucson -------------------- Strain: BA1091 Species: Caenorhabditis elegans Genotype: C35D10.2(hc192) III. Description: No visible phenotype in hermaphrodites. Primes HES 146/146 and HES 148/149. Mutagen: TMP Outcrossed: x6 Reference: Made by: HES Received: 10/23/01 from Ward S, University of Arizona, Tucson -------------------- Strain: BA1093 Species: Caenorhabditis elegans Genotype: snf-10(hc194) V. Description: Y32F6A.2 No visible phenotype in hermaphrodites. Primers: outer HES 22/228 and inner HES 229/230. Mutagen: TMP Outcrossed: x6 Reference: Made by: H. Smith Received: 10/15/01 from Ward S, University of Arizona, Tucson -------------------- Strain: BB1 Species: Caenorhabditis elegans Genotype: dcr-1(ok247)/unc-32(e189) III. Description: Heterozygotes are WT and segregate WT, Uncs, and Steriles. Mutagen: EMS Outcrossed: x8 Reference: CGC #4870 Knight SW;Bass BL A role for the RNase III enzyme DCR-1 in RNA interference and germ line development in Caenorhabditis elegans. Science 293: 2269-2271 2001 Made by: B. Barstead Received: 01/30/02 from Bass B, University of Utah, Salt Lake City, UT -------------------- Strain: BB2 Species: Caenorhabditis elegans Genotype: adr-1(gv6) I. Description: Defective chemotaxis to volatile odorant. Low percentage (about 6%) have protruding vulva. Mutagen: EMS Outcrossed: x8 Reference: CGC #5596 Tonkin LA;Saccomanno L;Morse DP;Brodigan T;Krause M;Bass BL RNA editing by ADARs is important for normal behavior in Caenorhabditis elegans. EMBO Journal 22: 6025-6035 2002 Made by: Michael Krause Received: 02/05/03 from Bass B, University of Utah, Salt Lake City, UT -------------------- Strain: BB3 Species: Caenorhabditis elegans Genotype: adr-2(gv42) III. Description: Defective chemotaxis to volatile odorant. Mutagen: EMS Outcrossed: x8 Reference: CGC #5596 Tonkin LA;Saccomanno L;Morse DP;Brodigan T;Krause M;Bass BL RNA editing by ADARs is important for normal behavior in Caenorhabditis elegans. EMBO Journal 22: 6025-6035 2002 Made by: Michael Krause Received: 02/05/03 from Bass B, University of Utah, Salt Lake City, UT -------------------- Strain: BB4 Species: Caenorhabditis elegans Genotype: adr-1(gv6) I; adr-2(gv42) III. Description: Defective chemotaxis to volatile odorant. Low percentage (about 6%) have protruding vulva. Mutagen: Outcrossed: x Reference: CGC #5596 Tonkin LA;Saccomanno L;Morse DP;Brodigan T;Krause M;Bass BL RNA editing by ADARs is important for normal behavior in Caenorhabditis elegans. EMBO Journal 22: 6025-6035 2002 Made by: Michael Krause Received: 02/05/03 from Bass B, University of Utah, Salt Lake City, UT -------------------- Strain: BC14838 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14838. Description: sEx14838[rCesC24B9.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (CGC#6525). Mutagen: Outcrossed: x0 Reference: Made by: Domena Tu Received: 05/14/07 from Baillie D, Simon Fraser University, Vancouver, Burnaby, BC -------------------- Strain: BC14839 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14839. Description: sEx14839[rCesC24B9.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (CGC#6525). Mutagen: Outcrossed: x0 Reference: Made by: Domena Tu Received: 05/14/07 from Baillie D, Simon Fraser University, Vancouver, Burnaby, BC -------------------- Strain: BC14840 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14840. Description: sEx14840[rCesF47D2.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Co