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Extradiol Dioxygenases |
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| Homoprotocatechuate 2,3-dioxygenase (HPCD) catalyzes the
cleavage of homoprotocatechuate (3,4-dihydroxylphenyl acetate). In
Brevibacterium
fuscum HPCD uses Fe(II) as a cofactor; in Arthrobacter
globiformis
CM-2 HPCD uses Mn(II) as a cofactor. The structures of HPCD from
both organisms has recently been determined by the Ohlendorf lab.
The amino acid sequences of these HPCD's are 81% identical
Work on HPCD is in collaboration with Dr. John Lipscomb and Dr. Larry Wackett of the Dept. of Biochemistry. |
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